| 猪饲料中喹噁啉类药物酶联免疫检测方法的建立 |
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| 引用本文： | 赵春保,彭大鹏,陶燕飞,陈冬梅,袁宗辉.猪饲料中喹噁啉类药物酶联免疫检测方法的建立[J].中国农业科技导报,2008,10(Z2):69-75. |
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| 作者姓名： | 赵春保 彭大鹏 陶燕飞 陈冬梅 袁宗辉 |
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| 作者单位： | 华中农业大学国家兽药残留基准实验室（HZAU）农业部食品安全评价重点开放实验室 |
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| 基金项目： | 国家食品安全重大科技专项项目
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| 摘 要： | 饲料中喹乙醇、卡巴氧等喹噁啉类药物的使用易导致在动物可食性组织的残留,具有致癌、致畸、致突变性,WHO、FAO和EU已禁止使用,我国也对其在动物肌肉和肝脏中的最大残留限量(MRL)做了规定,因此,有必要对此类药物进行监测。通过对包被原QCA-OVA、抗QCA-BSA抗体、羊抗兔HRP-IgG的工作浓度以及反应时间进行优化,建立了饲料中喹噁啉类药物的ELISA分析方法。ELISA方法的线性范围为0.2~625mg/L,IC50为40.3 mg/L,与喹乙醇、卡巴氧、痢菌净、喹烯酮和喹赛多等的交叉反应率分别为100%、244.2%、98.8%、319.6%和266.9%,猪饲料中喹噁类药物的LOD为1.46~7.38 mg/kg,回收率为71.0%~92.3%,批间变异系数为9.73%~20.0%。对5个厂家饲料样品检测结果与HPLC方法一致。本方法可检测饲料中的喹噁啉类药物残留,样品处理方法简单省时,结果灵敏可靠。
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| 关 键 词： | 饲料 喹噁啉类 酶联免疫 检测 |
Development of an ELISA Method for Detection of Five Derivatives of Quinoxaline-1,4-dioxide in Porcine Feeds |
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| ZHAO Chun-bao,PENG Da-peng,TAO Yan-fei,CHEN Dong-mei,YUAN Zong-hui.Development of an ELISA Method for Detection of Five Derivatives of Quinoxaline-1,4-dioxide in Porcine Feeds[J].Journal of Agricultural Science and Technology,2008,10(Z2):69-75. |
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| Authors: | ZHAO Chun-bao PENG Da-peng TAO Yan-fei CHEN Dong-mei YUAN Zong-hui |
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| Institution: | Huazhong Agricultural University|National Reference Laboratory of Veterinary Drug Residues（HZAU）|MOA Key Laboratory of Food Safety Evaluation|Wuhan 430070|China |
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| Abstract: | Olaquindox,carbadox and other derivatives of quinoxaline-1,4-dioxide can be accumulated in animal tissues and lead to cancer,abnormality and mutation when they were used in animal husbandry as feed additives.They have been banned by WHO,FAO and EU.Maximum residues limits(MRL) for animal muscle and liver tissues were also set up in China.So it is necessary to monitor the compounds used in feeds.An ELISA method was established to detect derivatives of quinoxaline-1,4-dioxide.Concentrations of OVA-QCA,antibody,and HRP-IgG were optimized and the reaction time was determined too.The range of detection was from 0.2 mg/L to 625 mg/L with inhibition concentration by 50%(IC50) was 40.3 mg/L.The cross-reactivity with olaquindox,cabadox,mequindox,quinocetone and cyadox was 100%,244.2%,98.8%,319.6% and 266.9%,respectively.The limits of detection(LOD) were from 1.46 mg/kg to 7.38 mg/kg,and the recoveries were from 71.0% to 92.3% with variation coefficients were from 9.73% to 20.0%.The results of detection for commercial feeds by present ELISA method were accordance with that by HPLC.This method can detect five derivatives of quinoxaline-1,4-dioxide,the sample preparation was simple and the results were reliable. |
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| Keywords: | feed olaquindox ELISA determination |
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