| 高效液相色谱法测定配合饲料中氯米芬的含量 |
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| 引用本文： | 战余铭,赵明晓,朱良智,刘华阳,李斌.高效液相色谱法测定配合饲料中氯米芬的含量[J].中国农业科技导报,2008,10(Z2):48-52. |
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| 作者姓名： | 战余铭 赵明晓 朱良智 刘华阳 李斌 |
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| 作者单位： | 农业部饲料质量监督检验测试中心（济南） 烟台市动物防疫监督所 |
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| 摘 要： | 用甲醇提取配合饲料中的氯米芬,提取液通过MCX小柱进行富集、净化,通过高效液相色谱仪进行检测,利用紫外检测器定量及二极管阵列检测器进行定性。实验表明,顺反式氯米芬分离良好,氯米芬浓度在0.5~25μg/mL范围内与峰面积呈线性相关,r=0.999 9;平均回收率为85%~95%;变异系数≤10%;反式氯米芬检测限为0.3 mg/kg,顺式氯米芬检测限为0.5 mg/kg;常用抗雌激素制剂在该条件下对氯米芬的测定均无干扰。
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| 关 键 词： | 配合饲料 氯米芬 高效液相色谱仪 固相萃取 |
Determination of Clomiphene in Compound Feed Using HPLC |
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| ZHAN Yu-ming,ZHAO Ming-xiao,ZHU Liang-zhi,LIU Hua-yang,LI Bin.Determination of Clomiphene in Compound Feed Using HPLC[J].Journal of Agricultural Science and Technology,2008,10(Z2):48-52. |
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| Authors: | ZHAN Yu-ming ZHAO Ming-xiao ZHU Liang-zhi LIU Hua-yang LI Bin |
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| Institution: | 1.Feed Quality Supervision and Testing Center（Ji nan）|Ministry of Agriculture|Ji nan 210036|2.Yantai Animal Epidemic Prevention Institute|Shandong Yantai 264000|China |
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| Abstract: | The clomiphene was extracted from compound feed by methanol.The extract was enriched and purified by MCX-SPE,and then tested by HPLC.Quantitative analysis was carried out by UV-detector and qualitative analysis was by DAD.The result showed that cis-clomiphene and trans-clomiphene could be well seperated,the concentration of clomiphene and peak area was linear correlation between 0.5~25μg/mL,r=0.999 9,the average reclaim ratio was 85%~95%,and coefficient of variation was not exceeding 10%.The LDL of cis-clom... |
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| Keywords: | compound feed clomiphene HPLC SPE |
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