| 盐度对金钱鱼幼鱼存活、生长和抗氧化系统的影响 |
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| 引用本文： | 孙雪娜,冯广朋,刘鉴毅,王妤,庄平,余焱方,邹雄.盐度对金钱鱼幼鱼存活、生长和抗氧化系统的影响[J].上海海洋大学学报,2022,31(1):161-169. |
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| 作者姓名： | 孙雪娜 冯广朋 刘鉴毅 王妤 庄平 余焱方 邹雄 |
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| 作者单位： | 中国水产科学研究院东海水产研究所,中国水产科学研究院东海水产研究所,中国水产科学研究院东海水产研究所,中国水产科学研究院东海水产研究所,中国水产科学研究院东海水产研究所,中国水产科学研究院东海水产研究所,中国水产科学研究院东海水产研究所 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金项目 |
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| 摘 要： | 为探讨盐度对金钱鱼(Scatophagus argus)幼鱼存活、生长和抗氧化状态的影响,在6个盐度梯度(淡水和盐度5、10、20、30和35)下,养殖体质量为(6.27±0.28)g金钱鱼幼鱼60 d,统计其存活率及生长情况,测定各盐度下金钱鱼幼鱼肝脏、鳃、肌肉组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽S-转移酶(GST)活力,以及还原型谷胱甘肽(GSH)和丙二醛(MDA)含量。结果表明:金钱鱼幼鱼在盐度为0~35时存活率为97.78%~100%,无显著性差异(P>0.05)。盐度为5时,终末体长、体质量和增重率均达到最大,盐度为35时,终末体长、体质量和增重率最小。盐度5条件下的特定生长率为(0.96±0.01)%,显著高于其他盐度组(P<0.05)。基于特定生长率的二次回归模型拟合,预测体质量为(6.27±0.28)g的金钱鱼幼鱼高效养殖盐度为7.5。淡水组和盐度35高盐组金钱鱼幼鱼肝脏中SOD与CAT活力显著高于其他各组(P<0.05),并且肝脏中的GPx活力和MDA含量也处于较高水平。GST活力与GSH含量在各盐度组间没有显著性差异(P>0.05)。研究揭示:金钱鱼幼鱼在0~35的盐度时具有高存活率,但在低盐环境中生长较快;淡水和高盐环境能引起鱼体内产生一定程度的抗氧化应激。推荐金钱鱼幼鱼养殖盐度为5~10。
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| 关 键 词： | 金钱鱼 盐度 存活 生长 抗氧化酶 |
| 收稿时间： | 2020/10/22 0:00:00 |
| 修稿时间： | 2021/4/26 0:00:00 |
Effects of salinity on survival, growth and antioxidant defense system of Scatophagus argus |
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| SUN Xuen,FENG Guangpeng,LIU Jianyi,WANG Yu,ZHUANG Ping,YU Yanfang,ZOU Xiong.Effects of salinity on survival, growth and antioxidant defense system of Scatophagus argus[J].Journal of Shanghai Ocean University,2022,31(1):161-169. |
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| Authors: | SUN Xuen FENG Guangpeng LIU Jianyi WANG Yu ZHUANG Ping YU Yanfang ZOU Xiong |
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| Institution: | East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China,East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China |
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| Abstract: | In order to study the effects of salinity on the survival, growth and antioxidant status of different tissues of juvenile Scatophagus argus, juveniles with average weight of (6.27±0.28) g were exposed to different salinities (0,5, 10, 20, 30, 35) for 60 days, and the survival rate and growth status were counted. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and the contents of glutathione (GSH) and malondialdehyde (MDA) in liver, gill and muscle tissues were determined. The results showed that the survival rate was 97.78±1.11%~100% in the range of 0~35 salinity, and there was no significant differences between the groups (P>0.05). When the salinity was 5, the final body length, body weight and weight gain rate reached the maximum, while the value reached the minimum when the salinity is 35. The specific growth rate under the condition of salinity 5 was 0.96±0.01%, which was significantly higher than all other groups (P<0.05). The optimal breeding salinity under the condition was salinity 7.5 obtained by the quadratic regression model fitting based on the specific growth rate. Liver is the main organ to resist oxidative stress. The activities of SOD and CAT in the liver of fresh water group and salinity 35 group were significantly higher than that in the other groups (P<0.05), the activity of GPx and the content of MDA in liver were also at a higher level. There was no significant differences in the activity of GST and GSH content among different salinities (P>0.05). The results showed that the survival rate of Scatophagus argus juveniles was high in the salinity range of 0~35, but there were differences in the growth at different salinities, juvenile Scatophagus argus grew slower in the hypertonic environment, while grew faster in the low permeability environment. The fresh water and hypertonic environment caused a certain degree of oxidative stress in fish. |
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| Keywords: | Scatophagus argus salinity survival growth antioxidant enzymes |
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