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| 枯草芽孢杆菌Czk1菌株对橡胶树根病菌的抑制作用及对炭疽病生防效果研究初报 | |
| 引用本文： | 赵璐璐,贺春萍,郑肖兰,樊兰艳,郑服丛.枯草芽孢杆菌Czk1菌株对橡胶树根病菌的抑制作用及对炭疽病生防效果研究初报[J].广西农业科学,2011,42(7):740-743. |
| 作者姓名： | 赵璐璐 贺春萍 郑肖兰 樊兰艳 郑服丛 |
| 作者单位： | 1. 海南大学环境与植物保护学院;中国热带农业科学院环境与植物保护研究所 2. 中国热带农业科学院环境与植物保护研究所;农业部热带农林有害生物入侵监测与控制重点开放实验室;海南省热带农业有害生物检测监控重点实验室;海南省热带作物病虫害生物防治工程技术研究中心：海南儋州571737 3. 海南大学环境与植物保护学院 |
| 基金项目： | 国家天然橡胶产业技术体系病虫害防控专家岗位项目，中央级公益性科研院所基本科研业务费专项项目，公益性行业(农业)科研专项项目 |
| 摘 要： | 【目的】测定枯草芽孢杆菌Czk1菌株对橡胶树根病菌的抑菌活性及对炭疽病的生防效果，为进一步开发应用该菌株奠定基础。【方法】通过室内平板对峙和离体叶片接种的方法，测定Czk1菌株对13个橡胶树根病菌和1个炭疽病菌的拮抗活性以及对炭疽病的生防效果。【结果】Czk1菌株对13个橡胶树根病菌和1个炭疽病菌具有强烈的拈抗作用，且持续接种20代后依然具有很强的抑菌活性；Czk1菌株对橡胶树炭疽病有较好的生防效果，其中在发病前期先喷洒拮抗菌液的效果最好。【结论】枯草芽孢杆菌Czk1菌株对橡胶树红根、褐根、紫根、白根、臭根和炭疽病菌等多种致病真菌都有强烈的拮抗作用，且有抗菌谱广、持久稳定等特点，具有较高的潜在生防利用价值。 |
| 关 键 词： | 枯草芽孢杆菌 树根病 炭疽病 拮抗作用 橡胶树 |
Effect of Bacillus subtilis strain Czk1 on different rubber root pathogens and in vitro control of Colletotrichum gloeosporioides on rubber leaf |
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| ZHAO Lu-lu,HE Chun-ping,ZHENG Xiao-lan,FAN Lan-yan,ZHENG Fu-cong.Effect of Bacillus subtilis strain Czk1 on different rubber root pathogens and in vitro control of Colletotrichum gloeosporioides on rubber leaf[J].Guangxi Agricultural Sciences,2011,42(7):740-743. | |
| Authors: | ZHAO Lu-lu HE Chun-ping ZHENG Xiao-lan FAN Lan-yan ZHENG Fu-cong |
| Institution: | 1 College of Environment and Plant Protection, Hainan University; 2Environment and Plant Protection Institute, China Academy of Tropical Agricultural Sciences; 3 Key Laboratory of Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests, Ministry of Agriculture; 4Hainan Key Laboratory of Agricuhural Pests Detection and Control; 5 Hainan Engineering Research Center for Biological Control of Tropical Crops Diseases and Insect Pests： Danzhou, Hainan 571737, China) |
| Abstract: | Objective]The present experiment was conducted to study the effect of Bacillus subtilis strain Czk1 on controlling 13 rubber root pathogen and Colletotrichum gloeosporioides in vitro. Method]Antagonistic activity of Czk1 on 13 rubber root pathogens and C. gloeosporioides was determined in vitro using method of agar disk diffusion and isolated leaf inoculation. Result ]Czk1 had strong antagonistic effects on all the 13 rubber root pathogens and C. gloeosporioides, aud its inhibitory effects lasted until 20^th generation. Czk1 showed better biocontrol effects on C. gloeosporioides, specially after its spray before disease stage. Conclusion]Bacillus subtilis strain Czk1 had strong antagonistic effects on most of the pathogenic fungi of rubber plant, viz., Ganoderma pseudoferreum, Phellinus noxius, Helicobasidium compactum, Rigidoporus lignosus, Sphaerostilbe repens Colletotricbum gloeosporioides. With its characteristic broad antibacterial spectrum, steadyness and sustainability, it has a great potential for utilization in biocontrolling rubber plant pathogens. |
| Keywords: | Bacillus subtilis root disease Colletotrichum gloeospodoides antagonistic effect rubber tree |
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