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甘肃滩羊血清酯酶和α-淀粉酶多态性分析
引用本文:李积友,李秉诚,刘霞,折富有,武广善,宋祖才,田世雄,赵承银.甘肃滩羊血清酯酶和α-淀粉酶多态性分析[J].甘肃农业大学学报,2001,36(4):369-373.
作者姓名:李积友  李秉诚  刘霞  折富有  武广善  宋祖才  田世雄  赵承银
作者单位:1. 甘肃省畜牧技术推广总站,
2. 甘肃农业大学动物医学院,
3. 景泰县畜牧工作站,
4. 皋兰县畜牧工作站,
5. 靖远县畜牧工作站,
摘    要:运用聚丙烯酰胺凝胶电泳技术(PAGE)对甘肃滩羊产区皋兰、景泰及靖远3293只滩羊的血清酯酶(Es)、α-淀粉酶(α-Amy)基因座多态性进行了检测分析。结果表明:甘肃滩羊Es基因座存在3种基因型Es++ 、 Es+- 、 Es-- ,它们由Es+、Es-两个共显性等位基因控制,其中Es+-基因型在3个群体中均占优势,频率分别为0.620 0(皋兰)、0.666 6(景泰)、0.730 0(靖远);在皋兰群体和景泰群体中,Es-基因占优势,基因频率分别为0.540 0和0.548 4,而在靖远群体中优势基因则为Es+,其基因频率为0.525 0。在α-Amy基因座上没有检测到多态性。

关 键 词:滩羊  血清酯酶  α-淀粉酶  多态性分析
文章编号:1003-4315(2001)04-0369-05

The Polymorphism of Gansu Tan Sheep on Es andα-Amyloci in Gansu province
LI Ji-you,LI Bin-cheng,LIU Xia,ZHE Fu-you,WU Guang-shan,SONG Zu-cai,TIAN Shi-xiong,ZHAO Cheng-yin.The Polymorphism of Gansu Tan Sheep on Es andα-Amyloci in Gansu province[J].Journal of Gansu Agricultural University,2001,36(4):369-373.
Authors:LI Ji-you  LI Bin-cheng  LIU Xia  ZHE Fu-you  WU Guang-shan  SONG Zu-cai  TIAN Shi-xiong  ZHAO Cheng-yin
Institution:LI Ji-you1,LI Bin-cheng1,LIU Xia2,ZHE Fu-you5,WU Guang-shan1,SONG Zu-cai3,TIAN Shi-xiong4,ZHAO Cheng-yin5
Abstract:Polyacrylamide gel electrophoresis(PAGE) was applied to study polymorphism of Es and α-Amy loci of 293 Tan sheep in Gaolan county, Jingtai county and Jingyuan county of Gansu provine. The results showed that the locus of Es appeared 3 genetypes Es++, Es+-, Es which was decided by Es+ and Es- genes. Among 3 genetypes,Es+- was superior in all populations, the genetype frequencies were 0.6200(Gaolan),0.6666(Jingtai) and 0.7300(Jingyuan) resp ectively. Es- was dominant genes in Gaolan population and Jingtai population whose frequencies were 0.5400 and 0.5484 respectively correlationship with 0.5250 Es+ in Jingyuan population which was relatively superior. Monomorphism was investigated on α-Amy locus.
Keywords:Tan sheep  Es  α-Amy  polymorphism
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