| 洗涤厂排污口可培养中度嗜热菌的多样性 |
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| 引用本文： | 叶学军,;邱宏端,;林新坚,;贾宪波,;陈济琛.洗涤厂排污口可培养中度嗜热菌的多样性[J].福建省农科院学报,2014(8):768-773. |
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| 作者姓名： | 叶学军 ;邱宏端 ;林新坚 ;贾宪波 ;陈济琛 |
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| 作者单位： | [1]福州大学生物科学与工程学院,福建福州350108; [2]福建省农业科学院土壤肥料研究所,福建福州350013 |
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| 基金项目： | 国家公益性行业（农业）科研专项（201303094-05）;福建省财政厅社会公益研究项目（2060302）;福建省科技计划项目---省属公益类科研院所基本科研专项（2009R10002-2）;福建省星火计划项目（2012S006i） |
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| 摘 要： | 为考查洗涤厂热废水排放口土壤在常年人为热环境下的可培养嗜热菌的多样性，采集洗涤厂热废水排放口附近的湿热泥土，应用不同培养基分离纯化泥土中的可培养嗜热菌，并对获得的纯培养细菌进行16S rDNA鉴定和系统发育相关分析。结果表明：分离纯化得到的29株不同表型的纯培养菌株均属于坚壁菌门芽孢杆菌纲，归属于5个属11个种，分别是地芽孢杆菌属 Geobacillus 3个种，芽孢杆菌属 Bacillus 3个种，无氧芽孢杆菌属 Anoxybacillus 3个种，解脲芽孢杆菌属Ureibacillus 1个种和高温放线菌属 Thermoactinomyces 1个种，属种多样性丰富，菌株资源具有潜在开发应用价值。
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| 关 键 词： | 洗涤厂 可培养中度嗜热菌 分离 鉴定 多样性 |
Diversity of Culturable Mesophilic Bacteria from Outfall Soil of Laundry Factory |
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| Institution: | YE Xue-jun, QIU Hong-duan, LIN Xin-jian, JIA Xian-bo, CHEN Ji-chen (1. College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, China; 2. Soil and Fertilizer Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China) |
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| Abstract: | To investigate the diversity of mesophilic bacteria resulting from constant thermal conditions , the soil sample was collected at outfall of thermal wastewater from laundry factory .The culturable mesophilic bacteria were isolated with different medium to obtain pure culture , identified by 16S rDNA and the data were analyzed in phylogenetic tree .The results showed that the 29 strains in pure culture were obtained from the thermal wastewater outfall soil with different phenotype .The strains were classified into Bacilli ,attributed to 5 genus and 11 species , including three species of Geobacillus ,three species of Bacillus ,three species of Anoxybacillus ,one species of Ureibacillus ,and one species of Thermoactinomyces .The diversity of species was rich ,and the resources of species would have potential in practical application . |
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| Keywords: | laundry factory culturable mesophilic bacteria isolation identification diversity |
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