| 菊属植物遗传多样性的RAPD分析 |
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| 引用本文： | 刘蕤,杨际双.菊属植物遗传多样性的RAPD分析[J].河北农业大学学报,2010,33(1). |
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| 作者姓名： | 刘蕤 杨际双 |
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| 作者单位： | 1. 河北农业大学,园林与旅游学院,河北,保定,071001
2. 河北农业大学,园艺学院,河北,保定,071001 |
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| 基金项目： | 河北农业大学留学回国人员科研启动基金(2004-897) |
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| 摘 要： | 采用RAPD分子标记技术分析了菊属10个野生种和12个栽培品种间的遗传关系和多样性。从50个随机引物中筛选出了14条引物,对供试材料的DNA进行扩增,共获得169条清晰可辨的谱带,多态位点比率为96.4%,多态性较高。POPgene32软件计算结果表明:种(品种)间相似系数变幅在0.195 4~0.565 6;平均有效等位基因数为1.515 8,平均Nei’s基因多样性指数为0.321 6,平均Shannon信息指数为0.479 4。并且菊属野生种的多态条带、多态位点比率、平均有效等位基因数(Ne)、平均Nei’s基因多样性指数(He)及Shannon信息指数值均高于栽培菊花,表明野生种的遗传多样性比栽培菊花丰富。根据Jaccard相似系数进行UPGMA聚类结果表明,"若狭滨菊"与栽培菊花关系较近,野菊和小红菊与栽培菊花亦较近.栽培菊花基本可以按照花径聚类。
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| 关 键 词： | 菊属 菊花 RAPD 遗传多样性 遗传关系 |
Genetic diversity of some Dendranthema spp. based on RAPD analysis |
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| LIU Rui,YANG Ji-shuang.Genetic diversity of some Dendranthema spp. based on RAPD analysis[J].Journal of Agricultural University of Hebei,2010,33(1). |
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| Authors: | LIU Rui YANG Ji-shuang |
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| Institution: | 1.College of Landscape Architecture and Tourism;Agricultural University of Hebei;Baoding 071001;China;2.College of Horticulture;China |
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| Abstract: | 10 wild species and 12 cultivars of Dendranthema were used for analyzing genetic diversity and relationship by RAPD-PCR technology.The fourteen primers screened from 50 random primers,could generate 169 fragments,of which 163 ones(96.4%) were polymorphic,showed high polymorphism in Dendranthema.As analyzed by POPgene32,the similarity coefficient between cultivars ranged from 0.195 4 to 0.565 6.The average value of effective number of alleles,Nei's gene diversity and Shannon's informa-tion index were 1.515 8... |
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| Keywords: | RAPD Dendranthema RAPD Genetic diversity Genetic relationship |
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