| 鸭梨肌动蛋白2基因克隆和表达分析 |
| |
| 引用本文： | 闫洪波,葛文雅,杨坤,程玉豆,关军锋.鸭梨肌动蛋白2基因克隆和表达分析[J].河北农业大学学报,2012,35(1):27-30. |
| |
| 作者姓名： | 闫洪波 葛文雅 杨坤 程玉豆 关军锋 |
| |
| 作者单位： | 1. 河北省农林科学院遗传生理研究所,河北石冢庄050051;河北省植物转基因中心,河北石家庄050051;河北经贸大学生物科学与工程学院,河北石家庄050061
2. 河北省农林科学院遗传生理研究所,河北石冢庄050051;河北省植物转基因中心,河北石家庄050051 |
| |
| 基金项目： | 河北省博士后择优支持资金;河北省农林科学院博士后资金;河北省科技支撑计划项目资助 |
| |
| 摘 要： | 根据其他植物肌动蛋白2基因(Actin2)的保守序列设计一对简并性引物,从鸭梨叶片中克隆到1个编码肌动蛋白的基因,命名为PbACT2。PbACT2基因cDNA全长为1 180bp,开放阅读框1 134bp,编码377个氨基酸。氨基酸比对分析表明,该基因编码的氨基酸与其他陆生植物肌动蛋白基因具有较高的同源性。研究证明,PbACT2在鸭梨不同器官和不同发育时期的表达基本一致,说明该基因的表达较为稳定。
|
| 关 键 词： | 鸭梨 肌动蛋白2 基因克隆 半定量分析 表达稳定性 |
Cloning and expression analysis of Actin2 gene in Yali pear |
| |
| YAN Hong-bo
,
GE Wen-ya
,
YANG Kun
,
CHENG Yu-dou
,
GUAN Jun-feng.Cloning and expression analysis of Actin2 gene in Yali pear[J].Journal of Agricultural University of Hebei,2012,35(1):27-30. |
| |
| Authors: | YAN Hong-bo GE Wen-ya YANG Kun CHENG Yu-dou GUAN Jun-feng |
| |
| Institution: | 1,2 (1.Institute of Genetics and Physiology,Hebei Academy of Agricultural and Forestry Science,Shijiazhuang 050051,China; 2.Plant Genetic Engineering Center of Hebei Province,Shijiazhuang 050051,China; 3.Biosence and Bioengineering School,Hebei University of Economics and Business,Shijiazhuang 050061,China) |
| |
| Abstract: | Degenerate primers were designed to clone the ACT2 gene in leaf of Yali pear based on the conserved sequences of the actin genes from other plants,and named PbACT2.The cDNA of PbACT2 was 1 180 bp and contained a 1 134 bp open reading frame encoding a 377-amino acids protein.The deduced amino acid sequence of PbACT2 shared high identity with other ACTs multiple alignment.The result indicated that PbACT2 expressed constitutively in different organs and development stage,and implied that the expression of PbACT2 is stable. |
| |
| Keywords: | Yali pear ACT2 gene cloning semi-quantity analysis expression stability |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
