| 落叶松体细胞胚胎发生过程中DNA甲基化模式变化分析 |
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| 引用本文： | 魏华丽,吴涛,杨文华,李万峰,张俊红,齐力旺,韩素英.落叶松体细胞胚胎发生过程中DNA甲基化模式变化分析[J].东北林业大学学报,2011,39(2):33-37. |
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| 作者姓名： | 魏华丽 吴涛 杨文华 李万峰 张俊红 齐力旺 韩素英 |
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| 作者单位： | 中国林业科学研究院林业研究所;中国林业科学研究森林生态环境与保护研究所; |
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| 基金项目： | 国家高技术研究发展计划“863”计划重点项目(2006AA100109); 国家重点基础研究发展计划“973”项目(2009CB119106)资助 |
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| 摘 要： | 通过MSAP技术检测落叶松体细胞胚胎发生过程中的DNA甲基化模式变化发现,体细胞胚胎发生的14个时期中,5'-CCGG-3'序列的胞嘧啶上发生重新甲基化事件最多在ABA诱导后的0.5～6h,最少在5～7d的早期单胚阶段;而去甲基化事件发生最多的是5～7d的早期单胚阶段,最少在14～21d的中期单胚到晚期单胚阶段.与原胚...
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| 关 键 词： | 落叶松 体细胞胚胎发生 DNA甲基化 模式变化 重新甲基化 去甲基化 |
DNA Methylation Pattern Changes During Somatic Embryogenesis of Larix |
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| Wei Huali,Wu Tao,Yang Wenhua,Li Wanfeng,Zhang Junhong,Qi Liwang,Han Suying.DNA Methylation Pattern Changes During Somatic Embryogenesis of Larix[J].Journal of Northeast Forestry University,2011,39(2):33-37. |
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| Authors: | Wei Huali Wu Tao Yang Wenhua Li Wanfeng Zhang Junhong Qi Liwang Han Suying |
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| Institution: | Wei Huali,Wu Tao,Yang Wenhua,Li Wanfeng,Zhang Junhong,Qi Liwang(Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,P. R.China),Han Suying(Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry) |
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| Abstract: | A study was conducted to detect DNA methylation pattern changes during 14 stages of somatic embryogenesis of Larix by methylation sensitive amplification polymorphisms(MSAP) technique.Results showed that de novo methylation happened mostly during 0.5-6.0 h after ABA induction and the least of de novo methylation occurred at 5-7 d during the early period of single embryo.However,most demethylation happened at 5-7 d during the early period of single embryo and the least of demethylation occurred at 14-21 d du... |
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| Keywords: | Larix Somatic embryogenesis DNA methylation Pattern changes de novo methylation Demethylation |
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