| 乌苏里拟鲿(Pseudobagrus ussuriensis)同工酶分析 |
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| 引用本文： | 潘伟志,尹洪滨,孙中武,葛学亮,柯冬蕾.乌苏里拟鲿(Pseudobagrus ussuriensis)同工酶分析[J].东北林业大学学报,2006,34(6):66-69. |
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| 作者姓名： | 潘伟志 尹洪滨 孙中武 葛学亮 柯冬蕾 |
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| 作者单位： | 1. 中国水产科学研究院黑龙江水产研究所,哈尔滨,150070
2. 东北林业大学 |
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| 摘 要： | 采用聚丙烯酰胺凝胶电泳方法,对黑龙江水系乌苏里拟鲿(Pseudobagrus ussuriensis)心、肝、肾、眼、肌肉和性腺共6种组织进行了9种同工酶(ADH、EST、G6PDH、GDH、IDH、LDH、MDH、POD、SOD)的分析。结果表明,乌苏里拟鲿EST、GDH、IDH、POD同工酶谱中存在不同程度的组织特异性,EST表现出明显的性别差异性。共记录19个基因位点,多态位点百分数P=21.05%,种群遗传偏离指数d=-1,该乌苏里拟鲿种群内部杂合子缺失较严重,偏离Hardy-Weinberg平衡,遗传多样性较低。
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| 关 键 词： | 乌苏里拟鲿 同工酶 聚丙烯酰胺凝胶电泳 遗传多样性 |
| 收稿时间： | 2006-01-04 |
| 修稿时间： | 2006-01-04 |
Isozyme of Pseudobagrus ussuriensis in Heilongjiang Water System |
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| Pan Weizhi,Yin Hongbin,Sun Zhongwu,Ge Xueliang,Ke Donglei.Isozyme of Pseudobagrus ussuriensis in Heilongjiang Water System[J].Journal of Northeast Forestry University,2006,34(6):66-69. |
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| Authors: | Pan Weizhi Yin Hongbin Sun Zhongwu Ge Xueliang Ke Donglei |
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| Institution: | Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin 150040, P. R. China ; Northeast Forestry University |
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| Abstract: | Nine isoenzymes in tissues of heart, liver, eye, muscle, kidney and gonad from Pseudobagrus ussuriensis were analysed using vertical polyacrylamide gel electrophoresis, including ADH, EST, G6PDH, GDH, IDH, LDH, MDH, POD and SOD. Results showed that tissue-speciflc distribution was found in each electrophoresis pattern of EST, GDH, IDH and POD, and EST showed a distinct dissimilitude between intersexual samples. 19 genetic loci were detected, the polymorphic loci percentage was 21.05% and the genetic departure index was - 1. It indicates that the interior heterozygosity of Pseudobagrus ussuriensis is seriously lost, and the population is not in Hardy-Weinberg equilibrium and exhibits a lower genetic variation. |
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| Keywords: | Pseudobagrus ussuriensis Isoenzymes Polyacrylamide gel electrophoresis Genetic variations |
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