| 资源冷杉遗传多样性的ISSR分析 |
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| 引用本文： | 张玉荣,罗菊春,喻锦秀.资源冷杉遗传多样性的ISSR分析[J].北京林业大学学报,2007,29(6):41-46. |
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| 作者姓名： | 张玉荣 罗菊春 喻锦秀 |
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| 作者单位： | 北京林业大学林学院;湖南省林业科学院;北京林业大学林学院;湖南省林业科学院 |
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| 摘 要： | 应用ISSR分子标记方法对采自湖南和广西的6个资源冷杉野生群体共243个个体进行了遗传多样性分析.8个ISSR引物共扩增到了108个位点，其中84个是多态性位点，总的多态位点百分率（PPL）为77.78%.Nei’s基因多样性指数（He）为0.234 4，Shannon信息指数（I）为0.376 4. 6个不同群体的遗传多样性水平差异较大，群体多态位点百分率在22.22%～70.37%之间，Nei’s基因多样性指数为0.092 0～0.219 5，Shannon信息指数为0.134 4～0.339 1.香菇棚群体和银竹老山群体的遗传多样性水平较高，舜皇山群体的遗传多样性最低.资源冷杉自然群体间的遗传分化系数Gst为0.377 7，说明自然群体间存在一定程度的遗传分化；群体间基因流Nm为0.411 9，相对较弱，可能对自然群体的遗传分化有一定影响.基于Nei’s遗传距离进行了UPGMA聚类分析，6个群体的个体按群体各自聚在一起.
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| 关 键 词： | 资源冷杉 ISSR 遗传多样性 |
| 文章编号： | 1000-1522（2007）06-0041-06 |
| 收稿时间： | 2006-09-11 |
| 修稿时间： | 2006年9月11日 |
Genetic diversity of the endangered plant Abies ziyuanensis detected by ISSR markers |
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| ZHANG Yu-rong,LUO Ju-chun,YU Jin-xiu.Genetic diversity of the endangered plant Abies ziyuanensis detected by ISSR markers[J].Journal of Beijing Forestry University,2007,29(6):41-46. |
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| Authors: | ZHANG Yu-rong LUO Ju-chun YU Jin-xiu |
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| Institution: | 1 College of Forestry, Beijing Forestry University, 100083, P. R. China; 2 Hunan Forestry Academy, Changsha, 410004, P. R. China. |
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| Abstract: | As a member of Pinaceae, Abies ziyuanensis is an endangered species in China. The genetic diversity of 243 individuals of six A. ziyuanensis populations from Hunan and Guangxi provinces was estimated using inter simple sequence repeat(ISSR) markers. The eight primers employed produced a total of 108 scorable amplified fragments, of which 84(77.78%) were polymorphic. Genetic diversity analysis showed that Nei’s genetic diversity (He) was 0.234 4 and Shannon’s genetic information index (I) was 0.376 4. The genetic diversity level of six populations was different largely. The percentage of polymorphic loci of six populations changed from 22.22% to 70.37%, and Nei’s genetic diversity and Shannon’s genetic information index changed between 0.092 0-0.219 5 and 0.134 4-0.339 1, respectively. The levels of genetic diversity of Xianggupeng population and Yinzhulaoshan population were higher than those of the other populations, the level of Shunhuangshan population was the lowest. Coefficient of genetic differentiation（Gst）among six populations was 0.377 7, indicating that natural population was genetically differentiated to a certain extent. Gene flow among A. ziyuanensis was relatively weak (Nm=0.411 9), which might have caused the genetic differentiations among the populations. UPGMA cluster analysis showed thatthe 243 individuals were clustered into six clusters corresponding to the six populations. |
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| Keywords: | Abies ziyuanensis inter-simple sequence repeat(ISSR) genetic diversity |
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