| 减毒鸡白痢沙门菌△aroA株的构建与致病性分析 |
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| 引用本文： | 程伊洛,邵华斌,罗青平,刘国平,张腾飞.减毒鸡白痢沙门菌△aroA株的构建与致病性分析[J].湖北农业科学,2016(23):6195-6199. |
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| 作者姓名： | 程伊洛 邵华斌 罗青平 刘国平 张腾飞 |
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| 作者单位： | 1. 长江大学动物科学学院，湖北 荆州 434025; 湖北省农业科学院畜牧兽医研究所，武汉 430064;2. 湖北省农业科学院畜牧兽医研究所,武汉,430064;3. 长江大学动物科学学院,湖北 荆州,434025 |
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| 基金项目： | 公益性行业﹙农业﹚科研专项经费项目﹙201303044﹚；国家现代农业产业技术体系技术专项基金﹙CARS-42-G11﹚；湖北省农业科学院青年科学基金项目﹙2013NKYJJ10﹚ |
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| 摘 要： | 以鸡白痢沙门氏菌c79-3强毒株为亲本,通过λ-red同源重组系统构建鸡白痢沙门菌aroA基因缺失株△aroA,并进行了测序验证和毒力试验。结果表明,aroA基因缺失株的生长速度较亲本株缓慢,且该缺失株具有良好的遗传稳定性;相比野生菌株,aroA基因缺失株的毒力发生了下降,雏鸡攻毒死亡率较亲本株低。本研究获得的减毒鸡白痢沙门氏菌aroA基因缺失株△aroA,为进一步研究鸡白痢沙门氏菌aroA基因的功能和后续减毒活疫苗开发奠定了基础。
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| 关 键 词： | 鸡白痢沙门氏菌 λ-red同源重组 aroA 基因 生物学特性 活疫苗 |
Construction and Pathogenicity Analysis of Attenuated Salmonella Pullorum aroA Mutant |
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| Abstract: | The △aroA mutant was constructed by the λ-red recombinant based on c79-3 virulent strain and was confirmed by PCR and sequencing. The growth characteristics and genetic stability of the mutant strain was tested. The results showed that the growth rate of △aroA mutant was slow than the parent strain and was stable. The pathogenecity tests on one day-old chicks. The results showed that compared with wild strains, the virulence of aroA gene deletion mutant was decreased, and the attack rate of chicken was lower than that of the parent strain. In conclusion,we successfully obtained △aroA mutant strain and this study provided basic data for further study of the functions of the aroA gene and live attenuated vaccine. |
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| Keywords: | Salmonella pullorum λ-red recombinant system aroA gene biological characteristic live vaccine vector |
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