| 新疆乌鲁木齐市宠物源葡萄球菌耐药性及其相关耐药基因的检测 |
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| 引用本文： | 轩慧勇,郭菲,夏利宁,姚晓慧.新疆乌鲁木齐市宠物源葡萄球菌耐药性及其相关耐药基因的检测[J].中国农业大学学报,2018,23(6):85-91. |
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| 作者姓名： | 轩慧勇 郭菲 夏利宁 姚晓慧 |
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| 作者单位： | 新疆农业大学动物医学学院 |
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| 基金项目： | 国家自然科学基金-新疆联合基金项目（U1503185） |
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| 摘 要： | 为了解新疆乌鲁木齐市宠物源葡萄球菌对临床常用抗菌药物的耐药情况及携带耐药基因的情况,采用琼脂稀释法对乌鲁木齐市某宠物医院分离鉴定的154株宠物源葡萄球菌进行临床常用10种抗菌药物耐药性检测,并用PCR方法检测葡萄球菌9种耐药基因:喹诺酮类(norA)、大环内酯-林可胺类(ermB)、四环素类(tetA、tetM)、β-内酰胺类(mecA、femA)和氟苯尼考类(cfr、fexA和fexB)。耐药性检查结果表明葡萄球菌对苯唑西林、四环素、左氧氟沙星和氟苯尼考的耐药率在50%以上,宠物源葡萄球菌多重耐药集中在4~5类,且耐药谱型多样化。基因检测结果显示fexA(31.2%)基因的检出率最高,其次是ermB(26.7%),未检出cfr、fexB和tetA基因。新疆乌鲁木齐市宠物源葡萄球菌普遍存在耐药和多重耐药情况,其中氟苯尼考耐药基因fexA检出率最高,揭示宠物源葡萄球菌耐药情况严重,应根据药敏试验及基因检测结果,合理选用抗菌药物,并加强对耐药菌的监测。
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| 关 键 词： | 宠物源葡萄球菌 抗菌药物 耐药 耐药基因 乌鲁木齐 |
| 收稿时间： | 2017/10/30 0:00:00 |
Detection of drug resistance and resistant genes in Staphylococcus from pets in Urumqi,Xinjiang |
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| XUAN Huiyong,GUO Fei,XIA Lining and YAO Xiaohui.Detection of drug resistance and resistant genes in Staphylococcus from pets in Urumqi,Xinjiang[J].Journal of China Agricultural University,2018,23(6):85-91. |
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| Authors: | XUAN Huiyong GUO Fei XIA Lining and YAO Xiaohui |
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| Institution: | College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China,College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China,College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China and College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China |
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| Abstract: | The aim of this study was to understand the resistance to antibacterial drugs and the florfenicol resistance genes of Staphylococcus strains derived from pets in Urumqi, Xinjiang.A total of 154 strains of staphylococci were isolated and identified from pets of a pet hospital in Urumqi.The minimum inhibitory concentrations of Staphylococcus from fecal samples were determined by agar dilution method to 10 selected antimicrobial drugs.And quinolones-resistance gene (norA), macrolide-linergenines-resistance gene (ermB), tetracycline-resistance genes (tetA and tetM), beta-lactam-resistance genes (mecA and femA) and florfenicol-resistance genes (cfr, fexA and fexB) were detected by PCR.The results showed that the ratio of staphylococcus strains resistant to oxacillin, tetracycline, levofloxacin and florfenicol was over 50%.The multidrug resistance test results revealed that the resistance strains were mainly resistant to 4-5 drugs, and had a wide range of spectrum.The detection rates of fexA was 31.2% and followed by ermB (26.7%).cfr, fexB and tetA genes weren''t detected.In conclusion, the results showed that resistance and multidrug resistance of pet derived Staphylococcus were existed widely and the detection rate of fexA resistance gene was the highest, suggesting that situation of pet source Staphylococcus resistance was serious.The rational use of antibiotics should be based on drug susceptibility testing and genetic testing results, and it was necessary to strengthen Staphylococcus drug-resistance monitoring. |
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| Keywords: | Staphylococcus derived from pets antimicrobial drugs drug resistance resistant genes Urumqi |
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