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A neutralizing antibody selected from plasma cells that binds to group 1 and group 2 influenza A hemagglutinins |
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| Authors: | Corti Davide Voss Jarrod Gamblin Steven J Codoni Giosiana Macagno Annalisa Jarrossay David Vachieri Sebastien G Pinna Debora Minola Andrea Vanzetta Fabrizia Silacci Chiara Fernandez-Rodriguez Blanca M Agatic Gloria Bianchi Siro Giacchetto-Sasselli Isabella Calder Lesley Sallusto Federica Collins Patrick Haire Lesley F Temperton Nigel Langedijk Johannes P M Skehel John J Lanzavecchia Antonio |
| Institution: | Institute for Research in Biomedicine, 6500 Bellinzona, Switzerland. |
| Abstract: | The isolation of broadly neutralizing antibodies against influenza A viruses has been a long-sought goal for therapeutic approaches and vaccine design. Using a single-cell culture method for screening large numbers of human plasma cells, we isolated a neutralizing monoclonal antibody that recognized the hemagglutinin (HA) glycoprotein of all 16 subtypes and neutralized both group 1 and group 2 influenza A viruses. Passive transfer of this antibody conferred protection to mice and ferrets. Complexes with HAs from the group 1 H1 and the group 2 H3 subtypes analyzed by x-ray crystallography showed that the antibody bound to a conserved epitope in the F subdomain. This antibody may be used for passive protection and to inform vaccine design because of its broad specificity and neutralization potency. |
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