| 刺五加叶多糖的分离纯化及免疫活性研究 |
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| 引用本文： | 孟繁磊,陈瑞战,魏春雁,宋志峰.刺五加叶多糖的分离纯化及免疫活性研究[J].特产研究,2011,33(1):12-15. |
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| 作者姓名： | 孟繁磊 陈瑞战 魏春雁 宋志峰 |
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| 作者单位： | 1. 吉林省农科院农业环境与资源研究中心,长春,130033
2. 长春师范学院,长春,130032 |
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| 摘 要： | 研究刺五加叶水溶性多糖结构信息和免疫活性。采用热水提取、乙醇沉淀、Sevag法脱蛋白后得到刺五加粗多糖，粗多糖经EDAE-SepharoseFastFlow离子交换柱和SephadexG-75凝胶过滤柱2次纯化，得到均-多糖组分ASP-2—1；ASP-2—1经三氟乙酸水解、1-苯基-3-甲基-5-吡唑啉酮（PMP）衍生化、毛细管区带电泳法（CZE）测定其单糖组成；高效凝胶渗透色谱法测定其纯度和分子量；体外脾淋巴细胞增值试验测定其免疫活性。从刺五加叶中分离纯化得到的均-多糖组分SPA-2—1主要由葡萄糖、阿拉伯糖、半乳糖、果糖、鼠李糖、木糖等6种单糖组成，摩尔比为27．33：8．92：3．03：1：7．59：20．51，其分子量为13．6KD；小鼠脾淋巴细胞增殖试验表明，SPA-2—1可显著促进脾淋巴细胞的增殖，刺五加叶多糖SPA-2—1具有较强的体外免疫活性。
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| 关 键 词： | 刺五加叶 多糖 分离纯化 免疫活性 |
Study on the Separation,Purification and Immunological Activity of the Polysaccharide from Leaves of Acanthopanacis Senticosi |
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| MENG Fan-lei,CHEN Rui-zhan,WEI Chun-yan,SONG Zhi-feng.Study on the Separation,Purification and Immunological Activity of the Polysaccharide from Leaves of Acanthopanacis Senticosi[J].Special Wild Economic Animal and Plant Research,2011,33(1):12-15. |
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| Authors: | MENG Fan-lei CHEN Rui-zhan WEI Chun-yan SONG Zhi-feng |
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| Institution: | 1 (1.Jilin Academy of Agricultural Sciences,Changchun 130033,China;2 Changchun Normal College,Changchun 130032,China) |
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| Abstract: | Structural information and immunological activity water-soluble polysaccharide from leaves of Acanthopanacis Senticosi were investigated.Crude polysaccharide was obtained from leaves of A.Senticosi after removing protein by hot water extraction,ethanol precipitation and Sevag.A homogeneous polysaccharide,named ASP-2-1,was separated and purified from the crude polysaccharide through EDAE-Sepharose Fast Flow Ion-exchange chromatograph and Sephadex G-75 gel filtration chromatography.The monosaccharide components of ASP-2-1 were determined by capillary zone electrophoresis(CZE) after trifluoroacetolysis and l-phenyl-3-methyl-5-pyrazolone(PMP) derivatization in tendorn order. The purity and molecular weight of ASP-2-1 were determined by HPGPC.The immunological activity of ASP-2-1 was menstruated with spleen lymphocytes of mouse in vitro experiment.The ASP-2-1 whose molecular weight was 13.6 BCD was mainly composed of glucose,arabinose,galactose,fructose,rhamnose and xylose with the mole ratio of 27.33:8.92: 3.03:1:7.59:20.51.ASP-2-1 showed proliferating effect on splenic lymphocyte markedly.The ASP-2-1 from leaves of A. Senticosi had strong immunological activity in vitro. |
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| Keywords: | Acanthopanacis senticosi Polysaccharide Separation and purification Immunological activity |
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