| 直接ELISA与PCR联合检测人粪便中的沙门氏菌 |
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| 引用本文： | 张晓燕,黄金林,焦新安,刘秀梵.直接ELISA与PCR联合检测人粪便中的沙门氏菌[J].扬州大学学报(农业与生命科学版),2003,24(4):13-15. |
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| 作者姓名： | 张晓燕 黄金林 焦新安 刘秀梵 |
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| 作者单位： | 1. 扬州职业大学,轻工系,江苏,扬州,225001
2. 扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009 |
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| 基金项目： | 江苏省"十五"科技攻关项目(BE2003307);上海市科技兴农重点攻关项目(2002341) |
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| 摘 要： | 采用直接酶联免疫吸附试验(ELISA)与PCR相结合的方法,对某市饮食行业健康人群的1 426份人粪便样品进行沙门氏菌检测,并与国家标准方法对比,直接ELISA法的敏感性和特异性分别达100%和97.2%,PCR法的敏感性和特异性均为100%.确定的沙门氏菌快速检测优化程序为对大量样品采用直接ELISA筛检,去除阴性样品,阳性样品采用PCR法作进一步鉴定;需要定型时则用国家标准方法.此法具有高度的特异性、敏感性和快速简便等优点.
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| 关 键 词： | 沙门氏菌 酶联免疫吸附试验 聚合酶链反应 检测 |
| 文章编号： | 1671-4652(2003)04-0013-03 |
| 修稿时间： | 2003年9月2日 |
COMBINATION OF DIRECT-ELISA AND PCR FOR RAPID DETECTION OF SALMONELLA IN HUMAN FECES SAMPLES |
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| ZHANG Xiao-yang,HUANG Jiu-lin,JIAO Xin-an,LIU Xiu-fan.COMBINATION OF DIRECT-ELISA AND PCR FOR RAPID DETECTION OF SALMONELLA IN HUMAN FECES SAMPLES[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2003,24(4):13-15. |
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| Authors: | ZHANG Xiao-yang HUANG Jiu-lin JIAO Xin-an LIU Xiu-fan |
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| Abstract: | In the spring physical examination for employees in the restaurant industry, 1 426 human feces samples were detected by the combination strategy of direct-ELISA and PCR method. Compared with the results of National Standard method, the sensitivity and specificity of direct ELISA was 100% and 97%, respectively, while those of PCR method reached both 100%. An opitimized rapid protocol for the detection of Salmonella was developed, that is, use direct-ELISA to screen the large number of samples first, and then use PCR to test the ELISA positive samples, the final step is, if needed, to use National Standard method to determine the serotypes of Salmonellae. This combined method was highly specific, sensitive and very quick. It provides an efficient tool to handle many samples in diseases prevention, animal quarantine, food and feed hygiene. This procedure ensures the accuracy and reliability of detecting results and saves lots of material and manpower, which would create great social and economic interest. |
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| Keywords: | Salmonella PCR ELISA rapid detection |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
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