| 山羊关节炎-脑炎ELISA方法的建立及其应用 |
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| 引用本文： | 相文华,吕晓玲.山羊关节炎-脑炎ELISA方法的建立及其应用[J].中国农业科学,1997,30(1):71-76. |
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| 作者姓名： | 相文华 吕晓玲 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所，哈尔滨 150001 |
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| 基金项目： | “八五”国家重点科技攻关项目 |
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| 摘 要： | 应用间接酶联免疫吸附试验（ELISA），检测了山羊关节炎-脑炎病毒（CAEV）试验感染山羊和自然感染山羊的血清抗体，确定了最适宜的反应条件。CAEV感染山羊血清终点滴度结果表明：ELISA比琼脂扩散试验（ID）敏感性高，对自然感染山羊检出率比ID高3%左右。CAE标准阳性血清和自然感染阳性血清预先被特异性抗原中和后的ELISA反应均呈阴性，从而证实了本方法的特异性。因此，本方法适于CAE的流行病学调查和监测工作。
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| 关 键 词： | 山羊关节炎-脑炎 间接酶联免疫吸附试验 |
Establishment and Application of Enzyme Linked Immunosorbent Assay for Detection of Caprine Arthritis Encephalitis |
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| Abstract: | An enzyme linked immunosorbent assay (ELISA) is described for the detection of antibodies to caprine arthritis encephalitis virus(CAEV) antibody. Serum samples from both goats experimentally and naturally infected with CAEV were tested. The optimal conditions for the ELISA to detect CAE antibody were determined. Tests proved that the ELISA is much more sensitive than immunodiffusion (ID) test in detection of CAE antibody and duration of antibody detected by ELISA was longer than that by ID. To prove its specificity, an inhibitory ELISA was conducted in which CAE positive sera pre treated with CAEV infected cells gave negative reactions. Therefore ELISA offered as an obviously simple, sensitive and valuable method for monitoring epidemics of the CAE. |
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| Keywords: | Caprine arthritis encephalitis Indirect enzyme linked immunosorbent assay |
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