| 石榴皮多酚组成分析及其抗氧化活性 |
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| 引用本文： | 李建科,李国秀,赵艳红,余朝舟.石榴皮多酚组成分析及其抗氧化活性[J].中国农业科学,2009,42(11):4035-4041. |
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| 作者姓名： | 李建科 李国秀 赵艳红 余朝舟 |
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| 摘 要： | 【目的】分析和研究石榴皮多酚的组成及其抗氧化活性。【方法】采用HPLC法分析石榴皮多酚提取物的组成及含量;采用体外抗氧化活性法测定多酚的还原能力、超氧阴离子自由基清除能力、羟自由基清除能力、DPPH?自由基清除能力和抗脂质过氧化能力;利用灌胃法研究石榴皮多酚提取物对小白鼠的血液、肝脏和脑组织中抗氧化指标的影响,评价其体内抗氧化活性。【结果】从石榴皮多酚提取物中鉴定出了9种多酚类成分,其中主要成分为安石榴苷,其含量占石榴皮总酚含量的65.75%。体外抗氧化活性试验表明,石榴皮多酚具有较强的还原能力、自由基清除能力和抗脂质过氧化能力。体内试验表明,石榴皮多酚能提高小鼠血液、肝脏及脑组织中 SOD活性和小鼠血液、肝脏中CAT、GSH-Px活性,降低小鼠血液、肝脏及脑组织中MDA含量,表明石榴皮多酚具有提高体内抗氧化酶系统的活性,从而增强机体抗氧化能力。【结论】石榴皮多酚含量丰富,其主要成分为安石榴苷,具有较强的抗氧化生物活性,是具有良好开发前景的天然抗氧化剂和保健食品及生物药品基料。
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| 关 键 词： | 石榴皮多酚 抗氧化活性 HPLC |
| 收稿时间： | 2009-02-25; |
Composition of Pomegranate Peel Polyphenols and Its Antioxidant Activities |
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| LI Jian-ke,LI Guo-xiu,ZHAO Yan-hong,YU Chao-zhou.Composition of Pomegranate Peel Polyphenols and Its Antioxidant Activities[J].Scientia Agricultura Sinica,2009,42(11):4035-4041. |
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| Authors: | LI Jian-ke LI Guo-xiu ZHAO Yan-hong YU Chao-zhou |
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| Institution: | (College of Food Engineering and Nutritional Science, Shaanxi Normal University)
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| Abstract: | Objective] The purpose of the experiment is to study the composition of pomegranate peel polyphenols (PPPs) and its antioxidant activities in vitro and in vivo. Method] The components and the contents of PPPs were analysed by HPLC, and the antioxidant activities in vitro of PPPs were measured by the methods of denxidization capability, superoxide anion radical scavenging capacity, hydroxyl radical scavenging capacity, DPPH radical scavenging capacity and anti-lipid peroxidation capacity. The effects of PPPs on antioxidant indexes of blood, liver and brain tissue in vivo were investigated by using mice as the experimental materials. Result] Nine phenolic compositions were identified from PPPs extracts, in which the pulicalagin is the main component with the content of 65.75% of total phenols. Antioxidant activities in vitro tests showed that PPPs had high deoxidization capacity, scavenging free radicals activities and anti-lipid peroxidation capacity. Animal experiments showed that PPPs could raise the SOD activities in blood, liver and brain tissue, as well as CAT, GSH-Px activities in blood and liver, but reduce the contents of MDA in blood, liver and brain tissue. Conclusion] Pomegranate peel are rich in polyphenols with the main component of pulicalagin, and it has significant antioxidant activities. It is concluded that PPPs may be exploited as a good natural antioxidant as well as an important base material of functional foods and biomedicinals. |
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| Keywords: | HPLC pomegranate peel polyphenols antioxidation activities HPLC |
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