| 基于GISH的甘蔗与蔗茅属间杂交F1后代染色体组成及核型分析 |
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| 引用本文： | 王先宏,杨清辉,李富生,何丽莲,何顺长.基于GISH的甘蔗与蔗茅属间杂交F1后代染色体组成及核型分析[J].中国农业科学,2011,44(6):1085-1091. |
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| 作者姓名： | 王先宏 杨清辉 李富生 何丽莲 何顺长 |
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| 作者单位： | (云南农业大学甘蔗研究所); |
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| 基金项目： | 国家自然科学基金，云南省人才培养计划项目，云南省现代农业甘蔗产业技术体系建设项目 |
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| 摘 要： | 【目的】探讨甘蔗(Saccharum spp.)与蔗茅(Erianthus fulvus)杂交F1材料的染色体组成及核型差异。【方法】以甘蔗与蔗茅间杂交所获得的16份F1材料为研究对象进行单色及双色基因组原位杂交(genomic in situ hybridization,GISH)研究,在GISH的基础上对3份材料的中期染色体进行核型分析。【结果】GISH的研究结果表明,子代材料中具有7—10条不等的父本染色体;由于母本遗传物质对父本遗传物质的排斥作用引起的染色体消减及片段化导致父本染色体在子代中有断裂的现象;研究结果表明,在间期核中亲本染色质以分散的方式单独分布;3份材料的中期染色体进行核型分析的公式分别为2n=92=80m(4SAT)+12sm、2n=88=82m+6sm及2n=88=78m(2SAT)+10sm,核型分类为2C、2C和2B。【结论】甘蔗×蔗茅杂种F1的染色体组成为n+n及2n+n,它们的核型均为较原始的染色体类型。
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| 关 键 词： | 甘蔗 蔗茅 F1子代 基因组原位杂交 核型 |
| 收稿时间： | 2010-07-18; |
Chromosomal Constitution and Karyotype of Intergeneric F1 Hybrids Between Saccharum spp.and Erianthus fulvus Based on GISH |
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| WANG Xian-hong,YANG Qing-hui,LI Fu-sheng,HE Li-lian,HE Shun-chang.Chromosomal Constitution and Karyotype of Intergeneric F1 Hybrids Between Saccharum spp.and Erianthus fulvus Based on GISH[J].Scientia Agricultura Sinica,2011,44(6):1085-1091. |
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| Authors: | WANG Xian-hong YANG Qing-hui LI Fu-sheng HE Li-lian HE Shun-chang |
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| Institution: | WANG Xian-hong,YANG Qing-hui,LI Fu-sheng,HE Li-lian,HE Shun-chang(Sugarcane Research Institute,Yunnan Agricultural University,Kunming 650201) |
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| Abstract: | 【Objective】The objective of current experiment was to study the chromosomal constitution and karyotype of F1 hybrids that derived from sexual cross between cultivated sugarcane (Saccharum spp.) and Erianthus fulvus.【Method】Both monocolor and bicolor GISH (Genomic in situ hybridization) were performed using digoxigenin-labeled E. fulvus DNA and biotin-labeled female parental DNA as probes, the karyotypes of 3 hybrids were analyzed based on the result of GISHs. 【Result】 The results of GISHs revealed the transmission of 7 to 10 E. fulvus chromosomes into progenies. The chromosomes of E. fulvus were broken because of the female parents prevented alien genetic materials from introgressing during distant hybridization and resulted in elimination and fragmentation of E. fulvus chromosomes. The GISH result on nuclear of progeny showed the chromatins came from male and female parent are not intermixed and stays at different regions. The karyotype formulas of 3 hybrids were 2n=92=80m (4SAT)+12sm. 2n=88=82m+6sm and 2n=88=78m(2SAT)+10sm,and their karyotypes were 2C, 2C and 2B, respectively. 【Conclusion】Chromosomal constitution of F1 hybrids that derived from sexual cross between cultivated sugarcane (Saccharum spp.) and E. fulvus were n+n and 2n+n. The results of karpotype analysis showed that the chromosomes of these three progenies are primitive. |
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| Keywords: | Saccharum spp Erianthus fulvus F1 hybrids GISH karyotype |
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