| 西藏普通小麦地方品种特有高分子量谷蛋白亚基组合“Tibetan Dx5*+Tibetan Dy10”中Tibetan Dy10亚基基因测序与分析 |
| |
| 引用本文： | 颜泽洪,代寿芬,刘登才,魏育明,郑有良.西藏普通小麦地方品种特有高分子量谷蛋白亚基组合“Tibetan Dx5*+Tibetan Dy10”中Tibetan Dy10亚基基因测序与分析[J].中国农业科学,2007,40(11):2403-2408. |
| |
| 作者姓名： | 颜泽洪 代寿芬 刘登才 魏育明 郑有良 |
| |
| 作者单位： | 四川农业大学小麦研究所,都江堰,611830 |
| |
| 基金项目： | 国家自然科学基金;教育部全国优秀博士学位论文作者专项基金;教育部跨世纪优秀人才培养计划;教育部长江学者和创新团队发展计划;国家高技术研究发展计划(863计划) |
| |
| 摘 要： | 【目的】鉴定在西藏小麦地方品种中发现的特有高分子量谷蛋白亚基组合“Tibetan Dx5*+Tibetan Dy10”中的Tibetan Dy10亚基是否与普通小麦Dy10亚基为同一亚基。【方法】利用SDS-PAGE分析和Tibetan Dy10亚基基因的克隆和测序。【结果】表明Tibetan Dy10亚基与普通小麦中Dy10亚基广泛存在的Dx5+Dy10组合形式中的Dy10亚基的分子序列非常相似，但分别在2个六肽中的1个氨基酸部位发生替换，第335位的甘氨酸（G）和第451位的谷氨酰氨（Q）在Tibetan Dy10 中均被替换为精氨酸（R）。【结论】Tibetan Dy10与普通小麦中常见的Dy10亚基基因的DNA序列存在微小差异，属于Dy10位点的一个新变异。
|
| 关 键 词： | 西藏小麦地方品种 稀有谷蛋白亚基组合 Dy10亚基 DNA测序 |
| 收稿时间： | 2006-10-31 |
| 修稿时间： | 2006-10-30 |
Sequencing Analysis of HMW Glutenin Subunit Gene Tibetan Dy10 in a Novel Subunit Combination of Tibetan Dx5*+Tibetan Dy10 in Tibetan Wheat Landrace |
| |
| YAN Ze-hong,DAI Shou-fen,LIU Deng-cai,WEI Yu-ming,ZHENG You-liang.Sequencing Analysis of HMW Glutenin Subunit Gene Tibetan Dy10 in a Novel Subunit Combination of Tibetan Dx5*+Tibetan Dy10 in Tibetan Wheat Landrace[J].Scientia Agricultura Sinica,2007,40(11):2403-2408. |
| |
| Authors: | YAN Ze-hong DAI Shou-fen LIU Deng-cai WEI Yu-ming ZHENG You-liang |
| |
| Institution: | 1Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City 611830 |
| |
| Abstract: | Sequencing and analysis of HMW glutenin subunit gene Tibetan Dy10 in a novel subunit combination of Tibetan Dx5*+Tibetan Dy10 in Tibetan wheat landrace
YAN Ze-hong, DAI Shou-fen, LIU Deng-cai, WEI Yu-ming, ZHENG You-liang
Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City, Sichuan province, China, 611830
Abstract: Objective] The objective of this study is focus on elucidating the main difference between HMW glutenin subunit gene Tibetan Dy10 in a novel HMW gluenin subunit combination of Tibetan Dx5*+Tibetan Dy10 in Tibetan wheat landrace and Dy10 in subunit combination of Dx5+Dy10 at molecular sequence level. Method] The SDS-PAGE analysis, cloning and sequencing method were used. Result] The result indicated that the molecular sequence of HMW glutenin subunit gene Tibetan Dy10 was very similar to that of Dy10 in HMW glutenin subunit combination Dx5+Dy10. The difference is that one amino acid in each of the two hexpeptide repetitive motifs in Dy10 has been substituted in Tibetan Dy10, respectively. Conclusion] Thought minor differences were found between Tibetan Dy10 and Dy10, there still have the reason that Tibeatan Dy10 is a Dy10 but not others.
Keywords: Tibetan wheat landrace; a novel HMW glutenin subunit combination; y-type HMW glutenin subunit; Sequencing |
| |
| Keywords: | Tibetan wheat landrace Novel HMW glutenin subunit combination Dy10 subunit DNA sequence |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
| 点击此处可从《中国农业科学》下载免费的PDF全文 |
|
