| 黄曲霉毒素B1降解菌株的筛选及鉴定 |
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| 引用本文： | 李俊霞,梁志宏,关舒,汪晓,王宁,计成,牛天贵.黄曲霉毒素B1降解菌株的筛选及鉴定[J].中国农业科学,2008,41(5):1459-1463. |
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| 作者姓名： | 李俊霞 梁志宏 关舒 汪晓 王宁 计成 牛天贵 |
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| 作者单位： | 李俊霞（中国农业大学食品科学与营养工程学院,北京,100083）;梁志宏（中国农业大学食品科学与营养工程学院,北京,100083）;关舒（中国农业大学动物科技学院,北京,100094）;汪晓（中国农业大学食品科学与营养工程学院,北京,100083）;王宁（中国农业大学动物科技学院,北京,100094）;计成（中国农业大学动物科技学院,北京,100094）;牛天贵（中国农业大学食品科学与营养工程学院,北京,100083） |
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| 摘 要： | 【目的】筛选能降解黄曲霉毒素B1（AFB1）的细菌，以期在该毒素的生物脱毒中得到应用。【方法】以香豆素为惟一碳源和能源进行AFB1降解菌株的初筛，之后将初筛的10株菌分别降解浓度为100μg?kg-1的AFB1。【结果】筛选出的NMO-3菌株降解AFB1能力达85.7%，显著高于其它菌株（P＜0.01）。从形态、生理生化反应以及16S rDNA序列比对等方面分析，最终确定NMO-3菌株为嗜麦芽窄食单胞菌（Stenotrophomonas sp.）。【结论】利用香豆素作为惟一碳源和能源筛选出了黄曲霉毒素降解菌株，后期试验证明活菌制剂在2.56×1010CFU/ml剂量以下不会引起急性毒性反应，用65%硫酸铵提取的蛋白（酶）具有AFB1降解能力。
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| 关 键 词： | 黄曲霉毒素B1 生物脱毒 嗜麦芽窄食单胞菌（Stenotrophomonas sp.） 鉴定 |
| 收稿时间： | 2007-5-15 |
| 修稿时间： | 2007年5月15日 |
Screening and Identification of Aflatoxin B1 Degradation Strains |
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| LI Jun-xia,LIANG Zhi-hong,GUAN Shu,WANG Xiao,WANG Ning,JI Cheng,NIU Tian-gui.Screening and Identification of Aflatoxin B1 Degradation Strains[J].Scientia Agricultura Sinica,2008,41(5):1459-1463. |
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| Authors: | LI Jun-xia LIANG Zhi-hong GUAN Shu WANG Xiao WANG Ning JI Cheng NIU Tian-gui |
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| Abstract: | Abstract：【Objective】The text was mainly to find the microorganism to degrade aflatoxin B1 and realized the application of biological degradation on AFB1．【Method】Using cumarin as the carbon source and energy，ten strains were first screened out of 1500，and the strain NMO-3 had high growth speed，its Morphology colony was big，the ability of utilizing cumarin was strong．We use aflatoxin B1 to have the second screening，through the ELISA method detecting AFB1 degradation ratio．【Results】The strain NMO-3 was screened out of ten strains， the degradation ratio of AFB1 reached 85．7%，it was prominent to the others（p﹤0．01）．With the analysis of colony morphology，physiological and biochemistry experiments，and 16SrDNA gene sequence，the strain NMO-3 was identified as Stenotrophomnas sp．【Conclusion】The results of this text showed that using cumarin as the carbon source and energy to screen out the AFB1 degradation strains was feasible．The text firstly reported the strain’s detoxification function． |
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