| 水稻抗白叶枯病基因Xa23的EST标记及其在分子育种上的利用 |
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| 引用本文： | 王春连,戚华雄,潘海军,李进波,樊颖伦,章琦,赵开军.水稻抗白叶枯病基因Xa23的EST标记及其在分子育种上的利用[J].中国农业科学,2005,38(10):1996-2001. |
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| 作者姓名： | 王春连 戚华雄 潘海军 李进波 樊颖伦 章琦 赵开军 |
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| 作者单位： | [1]中国农业科学院作物科学研究所／农业部作物遗传育种重点实验室 [2]农作物基因资源与基因改良国家重大科学工程,北京100081 [3]湖北省农业科学院粮食作物研究所,武汉430064 [4]国际水稻研究所中国办事处,北京100081 |
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| 基金项目： | 农业部“948”课题（2001-203）和国家“863”计划项目（2002AA2Z1001-05,2001AA211061）. |
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| 摘 要： | 用水稻抗白叶枯病基因Xa23的近等基因系CBB23与其感病轮回亲本JG30杂交，构建了包含2 562个单株的F2作图群体。抗性鉴定表明，F2植株抗感分离比严格符合3∶1。根据日本水稻基因组计划RGP的数据，筛选并合成12个EST标记的引物，进行亲本间多态性检测，找到2个在CBB23与JG30间有多态性的EST标记，C189和CP02662。用该标记对F2群体中的571个感病单株进行分子检测和连锁分析，结果表明这两个EST标记位于Xa23基因的两侧，C189靠着丝粒一侧，与Xa23的遗传距离为0.8cM；CP02662靠端粒一侧，与Xa23的遗传距离为1.3cM。将C189成功用于水稻分子育种实践，标记辅助选择的正确率接近100%，已培育出3个将Xa23基因与高产、优质、抗褐飞虱等性状聚合的水稻恢复系。
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| 关 键 词： | 水稻 Xa23 水稻白叶枯病 EST标记 分子育种 |
| 收稿时间： | 02 6 2005 12:00AM |
| 修稿时间： | 2005-02-06 |
EST-Markers Flanking the Rice Bacterial Blight Resistance Gene Xa23 and Their Application in Marker-Assisted Selection |
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| WANG Chun-lian, QI Hua-xiong, PAN Hai-jun, LI Jin-bo, FAN Ying-lun, ZHANG Qi, ZHAOKai-jun.EST-Markers Flanking the Rice Bacterial Blight Resistance Gene Xa23 and Their Application in Marker-Assisted Selection[J].Scientia Agricultura Sinica,2005,38(10):1996-2001. |
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| Authors: | WANG Chun-lian QI Hua-xiong PAN Hai-jun LI Jin-bo FAN Ying-lun ZHANG Qi ZHAOKai-jun |
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| Institution: | 1.Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences; 2.National Key Facility for Crop Resources and Genetic Improvement, Beifing 100081; 3.Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064; 4.IRRI-China Office, Beifing 100081 |
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| Abstract: | A F2 population, with 2 562 individuals, of JG30/CBB23 was constructed for molecular mapping of the bacterial blight resistance gene Xa23 in rice. Inoculation test of the F2 plants showed that the segregation ratio of resistant and susceptible fits to 3:1. Based on the amthors previous mapping of Xa23 gene, 12 EST markers from Rice Genome Program (RGP) database were surveyed in the susceptible F2 plants and two markers, C189 and CP02662, were found flanking the Xa23 gene, with genetic distance of 0.8cM and 1.3cM, respectively. These two EST markers were successfully used in rice breeding program. The results showed that efficiency of C189 marker-assisted selection (MAS) for Xa23 gene was almost 100%. Three elite hybrid rice restore lines with Xa23 gene and improved bacterial blight resistance have been developed. |
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| Keywords: | Rice Xa23 Rice bacterial blight EST MAS |
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