| 含CpG序列PRRSV ORF5基因真核质粒构建及其免疫原性的研究 |
| |
| 引用本文： | 温建新,李俊,周顺,任慧英,刘文华,邹玲,徐文,牛钟相,王金宝,吴家强.含CpG序列PRRSV ORF5基因真核质粒构建及其免疫原性的研究[J].中国农业科学,2008,41(5):1503-1510. |
| |
| 作者姓名： | 温建新 李俊 周顺 任慧英 刘文华 邹玲 徐文 牛钟相 王金宝 吴家强 |
| |
| 作者单位： | [1]山东农业大学动物科技学院,山东泰安271018 [2]山东省农业科学院畜牧兽医研究所,济南250000 [3]青岛农业大学动物科技学院,山东青岛266109 |
| |
| 摘 要： | 【目的】提高猪繁殖与呼吸综合征病毒（PRRSV）基因免疫的效果，构建含CpG基序和PRRSV ORF5的真核重组表达质粒CpG-pVAX1-ORF5。【方法】经酶切鉴定和序列测定，将重组质粒转染COS-7细胞，经间接免疫荧光试验证实CpG-pVAX1-ORF5可表达PRRSV GP5蛋白。免疫SPF小鼠，检测鼠的特异性抗体滴度、脾T淋巴细胞亚群数量（CD4+和CD8+）以及淋巴细胞增殖反应（MTT法）水平。【结果】本试验构建的含CpG基序真核重组表达质粒CpG-pVAX1-ORF5能诱导小鼠产生针对PRRSV的体液免疫与细胞免疫。【结论】CpG-ODN可提高PRRSV基因疫苗的免疫效果。
|
| 关 键 词： | 猪繁殖与呼吸综合征病毒 ORF5基因 DNA免疫 |
| 收稿时间： | 2007-11-22 |
| 修稿时间： | 2007年11月22 |
Study on Construction and Immunogenicity of Eukaryotic Expression Vector Containing PRRSV ORF5 and CpG Motifs |
| |
| WEN Jian-xin,LI Jun,ZHOU Shun,ReN Hui-ying,LIU Wen-hua,ZOU Ling,XU Wen,NIU Zhong-xiang,WANG Jin-bao,WU Jia-qiang.Study on Construction and Immunogenicity of Eukaryotic Expression Vector Containing PRRSV ORF5 and CpG Motifs[J].Scientia Agricultura Sinica,2008,41(5):1503-1510. |
| |
| Authors: | WEN Jian-xin LI Jun ZHOU Shun ReN Hui-ying LIU Wen-hua ZOU Ling XU Wen NIU Zhong-xiang WANG Jin-bao WU Jia-qiang |
| |
| Abstract: | The recombinant expression vector plasmid CpG-pVAX1-E was transfected into COS-7 cells by using lipofectamine methods.The ORF5 mRNA of transfected cells were detected by RT-PCR. The transient expression of PRRSV SD2 ORF5 nucleocapsid protein was detected by indirect immunofluorescence assay(IFA). In some transfected COS-7 cells, the green fluorescence was showed.The recombinant expression plasmid was inoculated into SPF mice.The antibody ies specific for E protein in serum were confirmed by ELISA. T lymphocyte proliferation test(MTT) were used to detect the number of CD4+ 、CD8 + and the T lymphocyte proliferation in peripheral blood of mice vaccinated. The results showed that the recombinant plasmid induced CpG-pVAX1-E could induce higher response of cellular immune response and specific humoral immune responses in mice . CpG-ODN was effective adjuvant which could significantly enhance the immunogenicity of PRRSV SD2 ORF5 DNA vaccine. |
| |
| Keywords: | Porcine reproductive and respiratory syndrome virus（PRRSV）SD2 Eukaryotic expression vector E Gene Immunogenicity |
| 本文献已被 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
| 点击此处可从《中国农业科学》下载免费的PDF全文 |
|
