| 内生细菌对番茄青枯病的控病作用及其抗菌谱 |
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| 引用本文： | 赵凯,肖崇刚,孔德英.内生细菌对番茄青枯病的控病作用及其抗菌谱[J].西南农业大学学报,2006,28(2):314-318. |
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| 作者姓名： | 赵凯 肖崇刚 孔德英 |
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| 作者单位： | 西南大学植物保护学院 重庆400716(赵凯，肖崇刚)，重庆出入境检验检疫局 重庆400020(孔德英) |
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| 摘 要： | 对初步筛选出的10株番茄青枯病菌的内生拮抗细菌,通过平板拮抗和盆栽控病试验进一步筛选具有较好防效的菌株。平板拮抗试验结果表明,其中5株内生拮抗细菌(01-144,01-189,TR 03-081,TR 03-108,TR 03-124)对番茄青枯病有良好防效。对上述5株内生拮抗细菌进行盆栽控病试验,结果显示,利用拮抗菌的去菌发酵液对番茄青枯病的防治效果明显优于菌悬液;再利用这5株内生拮抗细菌的无菌发酵液作平板拮抗试验,得到对青枯病菌有较强抑菌活性的TR 03-081菌株,而且研究发现该菌株的无菌发酵液对玉米小斑病、烟草赤星病、茄子褐纹病等病害的病原菌也具有较强的抑菌活性,表明该菌具有较广的抗菌谱,鉴定结果显示该菌株为枯草芽孢杆菌。
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| 关 键 词： | 番茄青枯菌 内生细菌 生物防治 抗菌谱 |
| 文章编号： | 1000-2642（2006）02-0314-05 |
| 收稿时间： | 12 26 2005 12:00AM |
| 修稿时间： | 2005-12-26 |
CONTROLLING EFFECT OF ENDOPHYTIC BACTERIA ON RALSTONIA SOLANNACEARUM AND ITS ANTIFUNGAL SPECTRUM |
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| ZHAO Kai ,XIAO Chong- gang ,KONG De- ying.CONTROLLING EFFECT OF ENDOPHYTIC BACTERIA ON RALSTONIA SOLANNACEARUM AND ITS ANTIFUNGAL SPECTRUM[J].Journal of Southwest Agricultural University,2006,28(2):314-318. |
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| Authors: | ZHAO Kai XIAO Chong- gang KONG De- ying |
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| Institution: | 1. College of Plant Protection, Southwest University, Chongqing 400716, China; 2. Chongqing Entry-Exit Inspection and Quarantine Bureau, Chongqing 400020, China |
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| Abstract: | Ten strains of endophytic bacteria were studied in am attempt to screen for strains with better controlling effect on tomato bacterial wilt(Ralstonia solanacearum).Five strains(01-144,01-189,TR 03-081,TR 03-108 and TR 03-124) showed satisfactory controlling effect in plate culture.They were then tested in a greenhouse trial.The results showed that the disease-controlling efficiency of their leavening was better than that of their suspension Another plate culture experiment with the leavening of the strains was conducted and TR 03-108 showed the best repressing activity against R.solanacearum.In addition,this strain was identified to have a wide antifungal spectrum,being effective in repressing Bipolaris maydis,Alternaria alternata and Phomopsisvexans.Bacterial identification indicated that TR 03-108 is Bacillus subtili. |
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| Keywords: | tomato bacterial wilt endophytic bacteria biological control antifungal spectrum |
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