| HP—PRRS纯化灭活疫苗免疫效力研究 |
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| 引用本文： | 唐艳林,宋桂才,李国江,何玉友,刘军,王复蒙.HP—PRRS纯化灭活疫苗免疫效力研究[J].吉林农业科技学院学报,2012(4):4-7. |
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| 作者姓名： | 唐艳林 宋桂才 李国江 何玉友 刘军 王复蒙 |
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| 作者单位： | 吉林农业科技学院动物医学学院;保定瑞普生物药业有限公司;吉林正业生物制品有限公司 |
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| 基金项目： | 吉林市重点科技计划项目(项目编号:201022110) |
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| 摘 要： | 为提高目前高致病性蓝耳病（HR—PRRS）灭活疫苗的免疫效果，将经Marc-145细胞培养收获的HP-PRRS病毒原液超滤浓缩50倍，再经灭活后用Sephamse 4 Fast Flow分子筛柱层析，收集完整的病毒粒子，加油佐剂制成纯化HP—PRRS灭活疫苗。纯化后疫苗的攻毒保护率达到415以上，血清抗体检测阳转率达86％以上，均高于同批病毒液制成的未纯化灭活疫苗，且差异显著。试验证明，HR—PRRS病毒液经浓缩纯化制成疫苗后，增强了免疫效果。
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| 关 键 词： | HR—PRRS灭活疫苗 浓缩纯化 攻毒保护率 抗体阳转率 |
Study on Immune Effect of HP-PRRS Inactivated Vaccine |
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| TANG Yanlin,SONG Guicai,LI Guojiang,HE Yuyou,LIU Jun,WANG Fumeng.Study on Immune Effect of HP-PRRS Inactivated Vaccine[J].Journal Of Jilin Agricultural Science And Technology College,2012(4):4-7. |
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| Authors: | TANG Yanlin SONG Guicai LI Guojiang HE Yuyou LIU Jun WANG Fumeng |
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| Institution: | 1.Jilin Agricultural Science and Technology University School of Animal Medicine,Jilin 132101; 2.Baoding Ruipu Bio - Pharmacy Co.,Ltd,Baoding 071000;3.Jilin Zhengye Biological Product Co.,Ltd,Jilin 132101) |
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| Abstract: | To improve purification and protective potency of HP- PRRS inactivated vaccine, HP- PRRS vires multiplying and collected from Marc - 145 cells was concentrated 50 times and then inactivated. Complete virion is separated and collected by chromatography with Sepharose 4 Fast Flow. Oil adjuvant was added to prepare purified in- activated vaccine. Protective potency of purified vaccine is above 4/5 and positive conversion rate of antibody is over 86 % that all higher than that of unpurified vaccine. The differences are significant. The experiment illustrated that vaccines through concentrating and purifying can enhanced immune efficacy and non- viral protein can be removed. |
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| Keywords: | HP- PRRS inactivated vaccine concentrating and purified protective potency positive conversion rate of antibody |
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