| 动物组织中抗菌药物残留的微生物学快速筛选法研究 |
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| 引用本文： | 伍金娥,范盛先,王玉莲,常超,宋士波,陈品,袁宗辉.动物组织中抗菌药物残留的微生物学快速筛选法研究[J].华中农业大学学报,2006,25(6):645-649. |
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| 作者姓名： | 伍金娥 范盛先 王玉莲 常超 宋士波 陈品 袁宗辉 |
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| 作者单位： | 华中农业大学国家兽药残留基准实验室/国家食品安全评价重点开放实验室,武汉,430070 |
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| 基金项目： | 国家“十五”重点攻关项目(2002514-14-02)资助 |
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| 摘 要： | 以巨大芽孢杆菌为受试菌,建立一种高度敏感的动物组织中抗菌药物残留快速筛选拭子法。拭子法对猪、鸡组织(肌肉、肝脏)青霉素、红霉素、新霉素、庆大霉素的检测限均低于我国规定的最高残留限量,其准确度、精确度及假阴性率等性能指标均符合筛选要求。本方法与国外同类方法(FAST试剂盒)对6种抗菌药物的最低检测限一致。
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| 关 键 词： | 可食性组织 抗菌药物 残留 微生物学筛选法 |
| 文章编号： | 1000-2421（2006）06-0645-05 |
| 收稿时间： | 03 20 2006 12:00AM |
| 修稿时间： | 2006-03-20 |
Fast Screening Methods for Antimicrobial Agents Residues in the Edible Tissue |
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| WU Jin-e,FAN Sheng-xian,WANG Yu-lian,CHANG Chao,SONG Shi-bo,CHEN Pin,YUAN Zong-hui.Fast Screening Methods for Antimicrobial Agents Residues in the Edible Tissue[J].Journal of Huazhong Agricultural University,2006,25(6):645-649. |
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| Authors: | WU Jin-e FAN Sheng-xian WANG Yu-lian CHANG Chao SONG Shi-bo CHEN Pin YUAN Zong-hui |
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| Institution: | National Reference Laboratory of Veterinary Drug Residues /MOA Key Laboratory of Food Safety Evaluation , Huazhong Agricultural University, Wuhan 430070,China |
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| Abstract: | A high sensitive method was developed to screen antimicrobial residues in food animal tissues which is based on the inhibition by the growth of Bacillus megaterium.The detected limit of this method for penicillin,chlortetracycline,erythromycin,neomycin,gentamycin in the tissues of swine and broiler(muscle,kidney and liver) is lower than or near MRLs established in China.Moreover,accuracy,precision and the false-negative results of this method meet the need of screening method.A comparison was made between the swab tests and the same kind of foreign swab tests,which gave quite similar results to 6 antimicrobial agents.It is concluded that the distinction between them was very low and the swab tests should be a good screening method developed in China for the detection of antimicrobial drugs residues in swine tissue and urine and should have enormous promise in China. |
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| Keywords: | edible tissue antimicrobial agent residues microbiological screening methods |
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