| 基于RAPD标记进行水稻籼粳分类研究 |
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| 引用本文： | 姜树坤,钟鸣,徐正进,张丽,马慧,刘少霞.基于RAPD标记进行水稻籼粳分类研究[J].沈阳农业大学学报,2006,37(4):639-641. |
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| 作者姓名： | 姜树坤 钟鸣 徐正进 张丽 马慧 刘少霞 |
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| 作者单位： | 1. 沈阳农业大学,辽宁省农业生物技术重点实验室,沈阳,110161
2. 辽宁省北方粳稻育种重点实验室,沈阳,110161 |
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| 摘 要： | 应用RAPD标记方法对30个水稻栽培品种进行了籼粳分类研究。从80个随机引物中筛选出了扩增出具有多态性的谱带的18个引物,并从中选出重复性高、稳定性好的S1004,S1012,S1019,S1048,S1049,S1053,S1063,S1070,S1075共9个引物进行反应。依据随机引物对不同品种水稻总DNA扩增出的特异性扩增条带,用Ne i’s公式计算30个水稻品种间的相似系数,并以UPMGA法进行聚类分析,其结果与品种系谱基本吻合,证明RAPD标记可应用于水稻的分类。
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| 关 键 词： | 水稻 籼粳分类 随机扩增多态性DNA |
| 文章编号： | 1000-1700(2006)04-0639-03 |
| 收稿时间： | 2005-01-19 |
| 修稿时间： | 2005年1月19日 |
Classification of Rice Cultivars with RAPD Molecular Markers |
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| JIANG Shu-kun,ZHONG ming,XU Zheng-jin,ZHANG li,MA hui,LIU Shao-xia.Classification of Rice Cultivars with RAPD Molecular Markers[J].Journal of Shenyang Agricultural University,2006,37(4):639-641. |
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| Authors: | JIANG Shu-kun ZHONG ming XU Zheng-jin ZHANG li MA hui LIU Shao-xia |
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| Institution: | 1 Key Laboratory of Agricultural Biotechnology of Liaoning Province, Shenyang 1 10161, China ; 2 Key Laboratory of Rice Breeding of Liaoning Province, Shenyang Agricultural University, Shenyang 110161, China |
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| Abstract: | The present study used RAPD markers to study the relation of 30 rice cultivars.In the course of PCR,9 primers were selected from 80 primers to screen the samples.These 9 primers have polymorphism,they are S1004,S1012,S1019,S1048,S1049,S1053,S1063,S1070,S1071.Based on the pair wise comparisons of amplification products the genetic distance was calculated using Nei 's genetic distance and a dendrogram was constructed using an unweighted pair group method with arithmetical averages(UPGMA).These 30 rice cultivars were divided into 2 groups.This result was identical with pedigree analysis and proved that RAPD markers could be used in classification of rice cultivars. |
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| Keywords: | rice classification of Indicia and Japonica RAPD marker |
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