| 缢蛏致病菌假单胞菌的分子生物学分析 |
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| 引用本文： | 曹梅,王兴强,阎斌伦,张庆起.缢蛏致病菌假单胞菌的分子生物学分析[J].安徽农学通报,2008,14(23):33-34. |
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| 作者姓名： | 曹梅 王兴强 阎斌伦 张庆起 |
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| 作者单位： | 淮海工学院江苏省海洋生物技术重点建设实验室,江苏连云港,222005;赣榆县海洋渔业技术指导站,江苏赣榆,222100 |
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| 基金项目： | 江苏省教育厅青蓝工程项目
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江苏省海洋生物技术重点建设实验室开放基金
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国家科技支撑计划重大项目
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| 摘 要： | 从患病缢蛏（Sinvnovacula constricta）中分离到一株病原菌，命名为1#菌株。采用传统方法提取菌株DNA，PCR扩增其16SrDNA基因片段、测序，得到该菌基因片段序列长度为1438bp。用MegAlign软件进行比对5种不同种细菌基因序列，构建进化树。16SrDNA产物序列分析结果表明：该菌株的16SrDNA的核苷酸序列与多株假单胞菌（Pseudomonas sp）的同源性为99％，在系统发育树上构成一个分支。在细菌系统发育分类学上，该菌株属于假单胞属，但与已定名的假单胞菌有一定的差异，与几种未定名的假单胞菌的亲缘关系最接近。对其分类地位的最后确定还需进一步的系统发育学分析。
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| 关 键 词： | 缢蛏 假单胞菌 16SrDNA |
Molecular Biological Analysis of a Pseudomonas sp from Pathogenic Sinvnovacula constricta |
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| Cao Mei et al..Molecular Biological Analysis of a Pseudomonas sp from Pathogenic Sinvnovacula constricta[J].Auhui Agricultural Science Bulletin,2008,14(23):33-34. |
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| Authors: | Cao Mei |
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| Institution: | Cao Mei et al. ( 1 Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang 222005, China; 2 Ganyu Fishery Technical Extension Center, Ganyu 222100, China) |
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| Abstract: | There was one pathogenic strain isolated from Sinvnovacula constricta,which was named 1#.The DNA was extracted with the tradition method.The 16SrDNA fragment was amplified from strain 1# by PCR.Its gene fragments contains 1438bp.Using the MegAlign software,the homologus sequences were compared and the molecular phylogenetic tree was constructed.The 16SrDNA sequence homology and phylogenetic analysis indicated that strain 1# shared 99% 16SrDNA sequence homology with Pseudomonas sp and these sequences constituted a branch in phylogenetic tree.In the phylogenetic framework of bacterial classification,the strain 1# belongs to Pseudomonas sp.But the results showed that the strain 1# was differentiated from any of the named Pseudomonas species.Based on the phylogenetic analysis,the strain 1# exhibited the highest levels of similarity to Pseudomonas sp.Therefore,their taxonomic placement needs more information on genes level. |
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| Keywords: | 16SrDNA |
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