| 阿特拉津降解菌FM326（Arthrobacter sp.）的分离筛选、鉴定和生物学特性 |
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| 引用本文： | 李明锐,祖艳群,陈建军,湛方栋,何永美,李元.阿特拉津降解菌FM326（Arthrobacter sp.）的分离筛选、鉴定和生物学特性[J].农业环境科学学报,2011,30(11). |
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| 作者姓名： | 李明锐 祖艳群 陈建军 湛方栋 何永美 李元 |
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| 作者单位： | 云南农业大学资源与环境学院,昆明,650201 |
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| 基金项目： | 云南省自然科学基金项目(2010CD058); 云南省教育厅科学研究基金项目(2010Y339) |
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| 摘 要： | 采集除草剂阿特拉津污染的土壤,通过直接涂布法和富集驯化培养分离法,分别获得6株和5株能够降解阿特拉津的细菌。通过降解效率和降解动态试验,筛选到1株高效降解阿特拉津的菌株FM326,该菌株能以阿特拉津为唯一的碳源和氮源生长,培养96h后对1000mg·L-1阿特拉津降解效率达到97%。通过生理生化鉴定和16SrDNA序列分析,菌株FM326鉴定为节杆菌属(Arthrobacter sp.)细菌。该菌株表现出最适生长温度30～35℃,最适生长pH值5～9,好氧生长的生长特性。
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| 关 键 词： | 阿特拉津 降解菌 节杆菌属 降解效率 |
Isolation, Screening and Identification of Atrazine-degrading Bacterium FM326(Arthrobacter sp.) |
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| LI Ming-rui,ZU Yan-qun,CHEN Jian-jun,ZHAN Fang-dong,HE Yong-mei,LI Yuan.Isolation, Screening and Identification of Atrazine-degrading Bacterium FM326(Arthrobacter sp.)[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2011,30(11). |
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| Authors: | LI Ming-rui ZU Yan-qun CHEN Jian-jun ZHAN Fang-dong HE Yong-mei LI Yuan |
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| Institution: | LI Ming-rui,ZU Yan-qun,CHEN Jian-jun,ZHAN Fang-dong,HE Yong-mei,LI Yuan* (Colloge of Resources and Environment,Yunnan Agricultural University,Kunming 650201,China) |
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| Abstract: | The triazine herbicide atrazine(2-chloro-4-ethylamino-6-isopropyl-amino-s-triazine) is one of the most used pesticides. Atrazine is principally used for control of certain annual broadleaf and grass weeds, primarily in corn but also in sorghum, sugarcane, and other crops. Although atrazine has relatively low persistency in soil, its remanets have been found in many higher concentrations even years after application due to widespread use. Microbial remediation is an effective and economic method to control t... |
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| Keywords: | atrazine degrading bacteria Arthrobacter sp degrading efficiency |
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