| 黑麦草抗氧化酶对Cd、Zn和Pb复合污染的响应 |
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| 引用本文： | 张军,王文科,耿雅妮,任雪盈,王周锋,曹书苗.黑麦草抗氧化酶对Cd、Zn和Pb复合污染的响应[J].农业环境科学学报,2018,37(6):1117-1124. |
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| 作者姓名： | 张军 王文科 耿雅妮 任雪盈 王周锋 曹书苗 |
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| 作者单位： | 长安大学旱区地下水文与生态效应教育部重点实验室;宝鸡文理学院陕西省灾害监测与机理模拟重点实验室 |
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| 基金项目： | 国家自然科学基金重点项目（41230314）；旱区地下水文与生态效应教育部重点实验室项目（310829151140，310829151141） |
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| 摘 要： | 为研究植物抗氧化酶活性与重金属复合污染之间的关系,通过土培正交实验考察重金属Cd(0、10、20、30、40、50 mg·kg~(-1))、Zn(0、150、250、350、450、550 mg·kg~(-1))、Pb(0、100、300、500、700、900 mg·kg~(-1))复合污染在不同胁迫时间(14、21、28、35 d)对黑麦草过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性的影响。结果表明,Cd对CAT和POD起激活作用,对SOD起抑制作用;Zn和Pb对CAT和POD起抑制作用,对SOD起激活作用。在重金属复合污染胁迫下,POD和SOD活性随着培养时间的延长而显著增加,在28 d和35 d时达到最大,分别为对照的260%和637.4%。考虑交互作用,SOD活性对Cd×Zn、Cd×Pb、Zn×Pb有显著响应(P0.05)。3种抗氧化酶中,SOD活性对重金属污染反应最敏感,可以作为表征重金属污染的指示指标。
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| 关 键 词： | 重金属 正交实验 抗氧化酶 黑麦草 |
| 收稿时间： | 2017/10/31 0:00:00 |
The response to the combined pollution of cadmium, zinc, and lead by the antioxidant enzyme activity of Lolium perenne L. |
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| ZHANG Jun,WANG Wen-ke,GENG Ya-ni,REN Xue-ying,WANG Zhou-feng and CAO Shu-miao.The response to the combined pollution of cadmium, zinc, and lead by the antioxidant enzyme activity of Lolium perenne L.[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2018,37(6):1117-1124. |
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| Authors: | ZHANG Jun WANG Wen-ke GENG Ya-ni REN Xue-ying WANG Zhou-feng and CAO Shu-miao |
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| Institution: | Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of Education, Chang''an University, Xi''an 710054, China;Shaanxi Key Laboratory of Disaster Monitoring and Mechanism Simulation, Baoji University of Arts and Sciences, Baoji 721013, China,Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of Education, Chang''an University, Xi''an 710054, China,Shaanxi Key Laboratory of Disaster Monitoring and Mechanism Simulation, Baoji University of Arts and Sciences, Baoji 721013, China,Shaanxi Key Laboratory of Disaster Monitoring and Mechanism Simulation, Baoji University of Arts and Sciences, Baoji 721013, China,Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of Education, Chang''an University, Xi''an 710054, China and Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region of Ministry of Education, Chang''an University, Xi''an 710054, China |
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| Abstract: | To study the relationship between plant antioxidant enzyme activity and combination heavy metal pollution, the effects of combination pollution by heavy metals Cd(0, 10, 20, 30, 40, 50 mg·kg-1), Zn(0, 150, 250, 350, 450, 550 mg·kg-1), and Pb(0, 100, 300, 500, 700, 900 mg·kg-1) on the activity of the enzymes catalase(CAT), peroxidase(POD), and superoxide dismutase(SOD) were investigated for different periods of stress(14, 21, 28, 35 d), based on orthogonal and interactive soil culture experiments. The results showed that CAT and POD were activated by Cd, whereas SOD was inhibited. CAT and POD were inhibited by Zn and Pb, whereas SOD was activated. Under the contamination stress of a combination of heavy metals, POD and SOD activities significantly increased with incubation time. In addition, at 28 and 35 d, POD and SOD activities were respectively 260% and 637.4% of those of the control. SOD activity significantly responded to the interactive effects of Cd×Zn, Cd×Pb, and Zn×Pb(P<0.05) and insignificantly responded to CAT and POD activities. The response of SOD activity was more sensitive than that of the other two antioxidant enzymes. Therefore, SOD activity can be used as an indicative index in the evaluation of the heavy metal contamination of soil. |
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| Keywords: | heavy metals orthogonal experiment antioxidant enzyme Lolium perenne L |
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