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贵阳市散养户猪、鸡源性大肠杆菌耐药性调查
引用本文:尹泽东,肖康,金志强,方英,吕世明.贵阳市散养户猪、鸡源性大肠杆菌耐药性调查[J].贵州大学学报(农业与生物科学版),2010,29(1):85-87.
作者姓名:尹泽东  肖康  金志强  方英  吕世明
作者单位:1. 贵州大学,动物科学学院,贵州,贵阳,550025
2. 贵阳市农业局,贵州,贵阳,550004
摘    要:为调查贵阳市散养户猪、鸡大肠杆菌的耐药情况,本文通过采集贵阳市农村散养的猪、鸡肛门拭子105份,经分离、鉴定得到96株大肠杆菌,再应用琼脂稀释法测定其对10种抗菌药物的MIC值。结果表明,96株大肠杆菌对10种药物的耐药率依次为:氟苯尼考(88.5%)、头孢噻呋钠(83.4%)、土霉素(82.3%)、阿莫西林(79.2%)、链霉素(76%)、磺胺嘧啶钠(75.0%)、恩诺沙星(71.9%)、环丙沙星(63.6%)、卡那霉素(61.4%)、庆大霉素(53.2%)。96株大肠杆菌均为多重耐药菌株,最少的对2种药物耐药,最多的对10种药物耐药。其结果说明,贵阳市散养户猪、鸡源大肠杆菌的耐药较严重。

关 键 词:大肠杆菌  耐药性  琼脂稀释法  抗菌药

An Investigation in the Antibiotic Resistance of E.Coli Isolated from Famers' Pig and Chicken in Guiyang
YIN Ze-dong,XIAO Kang,JIN Zhi-qiang,FANG Ying,LU Shi-ming.An Investigation in the Antibiotic Resistance of E.Coli Isolated from Famers' Pig and Chicken in Guiyang[J].Journal of Mountain Agriculture & Biology,2010,29(1):85-87.
Authors:YIN Ze-dong  XIAO Kang  JIN Zhi-qiang  FANG Ying  LU Shi-ming
Institution:YIN Ze-dong , XIAO Kang , JIN Zhi-qiang , FANG Ying, LU Shi-ming( 1. College of Animal Science, Guizhou University, Guiyang 550025, China; 2. Agriculture Bureau of Guiyang City, Guiyang 550004, Guizhou Province, China)
Abstract:To investigate the antibiotic resistance of E. coli isolated from pig and chicken in Guiyang City, a total of 105 feces samples were collected and 96 E. coli strains were isolated and indentified from all samples. The MIC values of the 96 strains to 10 antibiotics were measured with the method of solid agar dilution. Result demonstrated that the resistance to florfenicol, ceftiofur sodium, oxytetracycline, amoxycillin, streptomycin, sulfsdiazine soditim, enrofloxacin, ciprofloxacin, kanamyci, and gentamicin were 88.5%, 83.4%, 82. 3%,79.2%,76.0%, 75.0%, 71.9%, 63.6%, 61.4%, 53.2%, respectively. Most of the 96 strains were muhiple-antibiotic-resistant, the resistant spectrum range from two to 10 antibiotics. Therefore, the resistance situation to antibiotics of E. coli in famers' pig and chicken is considerable severe in Guiyang City.
Keywords:E  coli  antibiotic resistance  Solid agar dilution  antibiotics
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