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新疆短星火绒草不同极性提取物抑菌作用研究
引用本文:乔君霞,索菲娅,黄罗冬,马赟,钟珏.新疆短星火绒草不同极性提取物抑菌作用研究[J].云南农业大学学报,2013,28(1):83-88.
作者姓名:乔君霞  索菲娅  黄罗冬  马赟  钟珏
作者单位:1新疆大学 生命科学与技术学院,新疆 乌鲁木齐 830046;2新疆莎车县网通中学,新疆 莎车 844700;3云南大学 中草药生物资源研究所云百草实验室,云南 昆明 650091;4新疆教育科研所,新疆 乌鲁木齐 830092
摘    要: 新疆短星火绒草(Leontopodium brachyactis Gandog.)是一种具有代表性的火绒草属植物,在民间很早就被作为药用植物治疗疾病。为进一步开发利用新疆短星火绒草提供依据,本研究利用不同极性的提取剂提取新疆短星火绒草的有效成分,采用滤纸片法和二倍稀释法研究了6种不同提取物的体外抗菌作用,并对50%乙醇提取物进行了最低抑菌浓度(MIC)和最低杀菌浓度(MBC)的测定。结果表明:新疆短星火绒草具有很好的抑菌作用。水提物和50%乙醇提取物均具有较强的抑菌效果,50%乙醇提取物最低抑菌浓度MIC为0.0625g/mL最低杀菌浓度MBC为0.125g/mL,具有广谱性抑菌作用。

关 键 词:新疆短星火绒草  提取物  抑菌  比较分析  新疆

Comparison on Antibacterial Effects of Extracts Used Different Polarity Extraction Agents in Leontopodium brachyactis Gandog.
QIAO Jun-xia,SUO Fei-ya,HUANG Luo-dong,MA Yun,ZHONG Jue.Comparison on Antibacterial Effects of Extracts Used Different Polarity Extraction Agents in Leontopodium brachyactis Gandog.[J].Journal of Yunnan Agricultural University,2013,28(1):83-88.
Authors:QIAO Jun-xia    SUO Fei-ya  HUANG Luo-dong    MA Yun  ZHONG Jue
Institution:1College of Life Science and Technology,Xinjiang University, Urumqi 830046,China;2Wangtong Middle School Xinjiang Shache County,Shache 844700,China; 3Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, Yunnan University, Kunming 650091, China;4Xinjiang Education Research Institute,Urumqi 830092,China
Abstract:Leontopodium brachyactis Gandog. is resourceful and representative in Leontopodium plants. It is regarded as folk medicinal plants for long time. So the theoretical background and applied basis should be established. In this study, different polarity extractants containing 6 different extracting reagents were used to extract the active ingredients from L. brachyactis. To compare the antibacterial effects of different extracts, filter paper method and gradient dilution way were used. The results showed that L. brachyactishad broad antibacterial effects. The extractive extracted from larger polarity extraction agents had strong antibacterial effects, in which the water extracts and 50% ethanol extracts had the stronger antibacterial effects compared with the others. In addition, the 50% ethanol extracts of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were assayed and they were 0.0625g/mL and 0.125g/mL, respectively. And the huge potential medicinal value is indicated obviously.
Keywords:Leontopodium brachyactis Gandog    extract  antibacterial  comparison analysis  Xinjiang
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