| 三个鸡品系遗传关系的RAPD分析* |
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| 引用本文： | 霍金龙,苗永旺,李莲军,王荣平,叶朗惠,娄义洲,赵平,刘丽仙,潘伟荣,毕保良.三个鸡品系遗传关系的RAPD分析*[J].云南农业大学学报,2006,21(2):222-227. |
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| 作者姓名： | 霍金龙 苗永旺 李莲军 王荣平 叶朗惠 娄义洲 赵平 刘丽仙 潘伟荣 毕保良 |
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| 作者单位： | [1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南大学生物资源保护与利用国家重点实验室,云南昆明650091 [3]云南农业职业技术学院畜牧兽医系,云南昆明650212 |
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| 摘 要： | 为了从分子水平上阐明快羽系、绿壳蛋系和合成系3个鸡品系之间的遗传差异及其遗传关系,为进一步开展这3个鸡品系的杂交配套利用提供依据,本文采用RAPD标记对3个品系的血样进行了群体遗传关系分析。实验共筛选出27条随机引物,对3个鸡品系的池DNA进行了RAPD分析。27条引物共产生235种扩增片段,扩增产物片段的长度大小在277~2441bp范围内。利用电泳分析数据分别计算了3个品系群体之间的遗传相似系数和遗传距离指数。结果表明:快羽系与绿壳蛋系间的遗传关系最近;而快羽系与合成系间的遗传关系最远。
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| 关 键 词： | 鸡 品系 RAPD标记 遗传相似系数 遗传距离指数 |
| 文章编号： | 1004-390X（2006）02-0222-06 |
| 收稿时间： | 2005-10-11 |
| 修稿时间： | 2005-10-11 |
Analysis of the Genetic Relationship of Three Chicken Lines by Using RAPD Markers |
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| HUO Jin-long,MIAO Yong-wang,LI Lian-jun,WANG Rong-ping,YE Lang-hui,LOU Yi-zhou,ZHAO Ping,LIU Li-xian,PAN Wei-rong,BI Bao-liang.Analysis of the Genetic Relationship of Three Chicken Lines by Using RAPD Markers[J].Journal of Yunnan Agricultural University,2006,21(2):222-227. |
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| Authors: | HUO Jin-long MIAO Yong-wang LI Lian-jun WANG Rong-ping YE Lang-hui LOU Yi-zhou ZHAO Ping LIU Li-xian PAN Wei-rong BI Bao-liang |
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| Institution: | 1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China; 2. Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091 ,China; 3. Department of Husbandry and Veterinary, Yunnan Agriculture Vocation-Technical College, Kunming 650212, China |
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| Abstract: | To elucidate the genetic differences and genetic relationships among three chicken lines (Fast-feathering line, Green-shell egg line and Synthetic line)at genome DNA level and provide the basic data for utilization the heterosis between them, the RAPD assays were conducted in this study by using blood samples of the three chicken lines. 27 primers which had produced good polymorphic RAPD markers were selected from 95 random primers. 3 genomic DNA pools from the total genome DNA of the three chicken lines were assayed by using the primers screened and 235 reproducible amplified fragments which were 277~2441bp long were obtained. Average band sharing coefficient and genetic distance index among three chicken breeds were estimated according to RAPD polymorphic data. The results revealed that the genetic relationship between Fast-feathering line and Green-shell egg line was closer, whereas the genetic relationship between Fast-feathering line and Synthetic line was more distant than any other pair of the lines. |
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| Keywords: | chicken line RAPD genetic similarity index genetic distance index |
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