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| 一种适合杨树树皮的蛋白质提取方法 | |
| 引用本文： | 赵相涛,吕全,赵嘉平,马跃,陈亮,刘会香,张星耀.一种适合杨树树皮的蛋白质提取方法[J].山东农业科学,2012,44(7):121-123. |
| 作者姓名： | 赵相涛 吕全 赵嘉平 马跃 陈亮 刘会香 张星耀 |
| 作者单位： | 1. 山东农业大学植物保护学院/山东省林业有害生物防控工程技术研究中心/山东省农业微生物重点实验室,山东泰安,271018 2. 国家林业局森林保护学重点实验室/中国林业科学院森林生态环境与保护研究所,北京,100091 3. 北京林业大学林学院,北京,100083 |
| 基金项目： | 国家科技部基础性工作专项(2009FY210100)的部分研究内容 |
| 摘 要： | 双向电泳技术是进行蛋白质组分分离和功能鉴定的重要步骤。提取和制备高质量的蛋白质是进行双向电泳的首要前提。杨树树皮组织中含有大量的干扰物质,影响了蛋白质的提取和双向电泳的分离效果。为建立一种适合杨树树皮蛋白质的双向电泳程序,本实验以107杨为材料,采用改良的三氯乙酸-丙酮沉淀法提取杨树树皮蛋白质,经双向电泳和考马斯亮蓝染色检测,得到了理想的双向电泳图谱,为杨树与病原互作的蛋白质组学研究奠定了基础。 |
| 关 键 词： | 蛋白质组学 杨树树皮 双向电泳技术 |
A Protein Extraction Method Suitable for Poplar Bark |
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| ZHAO Xiang-tao , L Quan , ZHAO Jia-ping , MA Yue , CHEN Liang , LIU Hui-xiang , ZHANG Xing-yao.A Protein Extraction Method Suitable for Poplar Bark[J].Shandong Agricultural Sciences,2012,44(7):121-123. | |
| Authors: | ZHAO Xiang-tao L Quan ZHAO Jia-ping MA Yue CHEN Liang LIU Hui-xiang ZHANG Xing-yao |
| Institution: | ZHAO Xiang-tao , L(U) Quan , ZHAO Jia-ping , MA Yue , CHEN Liang , LIU Hui-xiang , ZHANG Xing-yao |
| Abstract: | Two-dimensional electrophoresis(2-DE) is an important step for protein separation and function identification.Extracting and preparing high-quality protein is the first premise of 2-DE.There is a large amount of interfering substances in poplar bark tissues,which influence the extraction of protein and separation effect of 2-DE.In order to develop a 2-DE procedure suitable for poplar bark protein,the poplar 107 was used as material.The improved method of trichloroacetic acid(TCA)-acetone was adopted to extract the proteins of poplar bark.After the two-dimensional electrophoresis and detection with Coomassie brilliant blue,the desirable gel maps were obtained.It layed the foundation for proteomics research of poplar and pathogen interaction. |
| Keywords: | Proteomics Poplar bark Two-dimensional electrophoresis |
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