| 小麦成熟胚组织培养体系优化及优良转化受体基因型的筛选 |
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| 引用本文： | 陈俊男,高润红,邓志英,田纪春.小麦成熟胚组织培养体系优化及优良转化受体基因型的筛选[J].山东农业科学,2010(11):1-5. |
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| 作者姓名： | 陈俊男 高润红 邓志英 田纪春 |
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| 作者单位： | 国家作物生物学重点实验室/山东农业大学小麦品质育种研究室;上海市农业科学院作物育种栽培研究所; |
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| 基金项目： | 国家转基因生物新品种培育科技重大专项项目，国家转基因生物新品种培育科技重大专项项目 |
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| 摘 要： | 为建立适于优良品种遗传转化的高效组织培养体系,本研究以3种山东省推广小麦品种（济麦19、良星99、鲁麦14）和模式品种Bobwhite为材料,以成熟胚为外植体对其愈伤组织进行诱导和分化,探讨了2,4-D不同浓度下的3种诱导培养基（MS、N6、MSB5）和4种激素配比的分化培养基对小麦成熟胚离体培养的影响。结果表明,N6培养基的诱导效果最好,平均诱导率超过了80%,且当2,4-D的浓度为4 mg/L时诱导效果最佳。4种激素配比均可诱导小麦成熟胚愈伤组织分化,但分化率较低且处理之间差别较大,添加0.5mg/L IAA和2 mg/L ZT的MS培养基的平均分化率最高,为22.58%。小麦基因型的差别主要影响其分化率的高低而对诱导率的影响不大,3个品种中用于转化的最佳品种为鲁麦14。
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| 关 键 词： | 小麦成熟胚 愈伤组织 诱导 分化 |
Optimization of Regeneration System of Tissue Culture from Mature Embryos and Selection of Suitable Transformation Acceptor Genotype in Wheat |
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| CHEN Jun-nan,GAO Run-hong,DENG Zhi-ying,TIAN Ji-chun.Optimization of Regeneration System of Tissue Culture from Mature Embryos and Selection of Suitable Transformation Acceptor Genotype in Wheat[J].Shandong Agricultural Sciences,2010(11):1-5. |
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| Authors: | CHEN Jun-nan GAO Run-hong DENG Zhi-ying TIAN Ji-chun |
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| Institution: | CHEN Jun-nan1,GAO Run-hong2,DENG Zhi-ying1,TIAN Ji-chun1(1.State Key Laboratory of Crop Biology/Group of Quality Wheat Breeding of Shandong Agricultural University,T aian 271018,China,2.Institute of Crop Sciences of Shanghai Academy of Agricultural Sciences,Shanghai 201161,China) |
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| Abstract: | In order to establish an efficient regeneration system of tissue culture for genetic transformation of improved varieties,the mature embryos of 3 wheat cultivars(Jimai19,Liangxing 99,Lumai 14) planted in Shandong Province and model variety Bobwhite were used as explants to study the callus induction and differentiation;and the effects of 3 induction media(including 3 concentrations of 2,4-D) and 1 differentiation medium(including 4 ratios of hormone)on mature embryos in vitro culture were investigated.The r... |
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| Keywords: | Wheat mature embryo Callus Induction Differentiation |
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