| 一种新的非酶法分离荠幼胚的方法 |
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| 引用本文： | 洪亚平,张亚冰,吴国锋,赵文娟,阎婵婵.一种新的非酶法分离荠幼胚的方法[J].农业科学与技术,2009,10(3):36-38. |
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| 作者姓名： | 洪亚平 张亚冰 吴国锋 赵文娟 阎婵婵 |
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| 作者单位： | 河南科技大学农学院,河南洛阳,471003 |
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| 基金项目： | 国家自然科学基金，河南科技大学SRTP项目 |
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| 摘 要： | 1材料与方法1．1材料试验所用荠的花和幼嫩果实，于2009年2月下旬至3月上旬采自河南省洛阳市。采集后，在花序中、上部选取花已开败的幼嫩果实进行解剖，或将其浸泡于60％的乙醇溶液中备用。解剖前取出，再将其浸泡于蒸馏水（或自来水）中除去乙醇，以减缓花、果在解剖过程中的干缩进程。
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| 关 键 词： | 非酶法 荠幼胚 方法 幼嫩果实 |
A New Non-enzymatic Method to Isolate Immature Embryos of Capsella bursa-pastoris (L.) Medic. |
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| HONG Ya-ping,ZHANG Ya-bing,WU Guo-feng,ZHAO Wen-juan,YAN Chan-chan.A New Non-enzymatic Method to Isolate Immature Embryos of Capsella bursa-pastoris (L.) Medic.[J].Agricultural Science ＆ Technology,2009,10(3):36-38. |
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| Authors: | HONG Ya-ping ZHANG Ya-bing WU Guo-feng ZHAO Wen-juan YAN Chan-chan |
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| Institution: | ( College of Agronomy, Henan University of Science and Technology, Luoyang 471003) |
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| Abstract: | Objective] The aim of this study was to manually isolate the microstructures such as ovule and embryo of Capsella bursa-pastoris (L.) Medic. by the non-enzymatic method. Method] With the gum block and dissecting microscope, its floral characteristics, ovule and embryo were manually isolated and observed by the non-enzymatic method. Result] C. bursa-pastoris had four calyces, four petals, tetradynamous stamen and silicle, while its embryogenesis contained globular embryo, heart-shaped embryo and torpedo-shaped embryo. Conclusion] The microstructures such as ovule and embryo of plants become easier to be isolated manually with the viscosity of gum block, which has small damage on embryo or disadvantages of keeping original shapes, and also can used in multi fields studies. This test can also be accomplished in labs with poor experiment facilities. |
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| Keywords: | Capsella bursa-pastoris (L ) Medic Ovule Embryo Gum block Isolation |
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