| 圆鼻巨蜥遗传多样性的RAPD分析（摘要） |
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| 引用本文： | 傅美兰,于冬梅,王莹,彭建军.圆鼻巨蜥遗传多样性的RAPD分析（摘要）[J].农业科学与技术,2011(3):400-404. |
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| 作者姓名： | 傅美兰 于冬梅 王莹 彭建军 |
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| 作者单位： | 暨南大学生命科学技术学院;华南濒危动物研究所物种鉴定中心 |
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| 基金项目： | Supported by Natural Science Foundation of Guangdong Province( 9151026001000003 );Outstanding Young Scientists Fund of Guangdong Province Academy ( 200704 );Wildlife Conservation and Utilization Public Laboratories Fund of Guangdong Province(200901)~~ |
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| 摘 要： | 目的]采用RAPD技术对圆鼻巨蜥（Varanus salvator）进行遗传多样性分析。方法]用20个随机引物对圆鼻巨蜥36个个体的基因组DNA进行PCR扩增。结果]有10对引物能扩出清晰稳定的条带,共扩增出2952条DNA片段,平均每个个体扩增出82个条带,其中47条具多态性,多态性位点比为57.32%,36个个体间遗传距离为0.0359～0.3359,平均遗传距离为0.13592。Neis基因多样性指数H=0.1819,Shannons多样性指数I=0.2630,表明圆鼻巨蜥具有较高的遗传多样性。结论]采用UPGMA法构建了36个个体相互关系的分子聚类图,发现没有形成明显的种群分化。
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| 关 键 词： | 圆鼻巨蜥 遗传多样性 RAPD |
RAPD Analysis for Genetic Diversity of Varanus salvator |
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| FU Mei-lan,YU Dong-mei,WANG Ying,PENG Jian-jun.RAPD Analysis for Genetic Diversity of Varanus salvator[J].Agricultural Science ＆ Technology,2011(3):400-404. |
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| Authors: | FU Mei-lan YU Dong-mei WANG Ying PENG Jian-jun |
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| Institution: | 1. Life Science and Technology Institute,Ji’nan University,Guangzhou 510632; 2. Identification Centre for Wildlife Species,South China Institute of Endangered Animals,Guangzhou 510260 |
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| Abstract: | Objective] The aim was to carry out RAPD analysis on genetic diversity of Varanus salvator. Method] 20 random primers were used for PCR amplification of genomic DNA of 36 individuals of V. salvator. Result] 10 primers could produce highly reproducible RAPD bands. A total of 2 952 DNA fragments were successfully amplified. Each individual got 82 amplified bands on average,47 of which showed polymorphism. The polymorphic locus percent was 57.32%. The genetic distance among 36 individuals ranged from 0.035 9 to 0.335 9 with an average of 0.135 9. The Nei’s gene diversity index (H) and Shannon’s information index (I) were 0.181 9 and 0.263 0,respectively,indicating that V. salvator had greater genetic diversity. Conclusion] The phylogenetic tree was inferred by using UPGMA analysis,it was found that the 36 individuals could be classified as one group,and there was no obvious population differentiation. |
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| Keywords: | Varanus salvator Genetic diversity RAPD UPGMA analysis |
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