| Abstract: | Objective] The cold shock method was used to induce diploid gynngenesis of Silurus astus. Mthod] The Silurus astus sperms were irradia-ted by ultraviolet, then conducted for fertilization. The different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus as-tus. Result] Under such inducing condition that ultraviolet irradiated 15 min,cold shock started 5 min after fertilization, and cold shock duartion was 40 min,the survival rate of diploid Silurus astus reached the highest(8.5% ). Conclusion] The experiment laid foundation for culturing new excellent Silu- rus astus variety and accumulated original files for further study of gynogenesis development mechanism. |
