| 红锥、白椎、大叶栎3个树种分子鉴定及亲缘关系的ISSR分析 |
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| 引用本文： | 刘海龙,陈晓明,覃子海,杨开太,林建新,黄金使.红锥、白椎、大叶栎3个树种分子鉴定及亲缘关系的ISSR分析[J].农业科学与技术,2011(12):1781-1783. |
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| 作者姓名： | 刘海龙 陈晓明 覃子海 杨开太 林建新 黄金使 |
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| 作者单位： | 广西壮族自治区林业科学研究院;国家林业局中南速生材繁育实验室;广西优良用材林资源培育重点实验室 |
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| 基金项目： | Supported by the Fundamental Research Fund in Guangxi Academy of Forestry " Population Genetics Study of Castanopsis hystrix"(Forestry 200901)~~ |
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| 摘 要： | 目的]进行红锥、白椎、大叶栎3个树种分子鉴定。方法]利用ISSR-PCR方法构建红锥（Castanopsis hystrix）、白椎（Castanopsis carlesii Hayata）、大叶栎（Quercus griffithii Hook）3个树种的DNA指纹图谱,根据各个体的Nei氏遗传距离矩阵,利用UPGMA法进行聚类分析。结果]从50个ISSR引物中筛选出6个多态性引物用于正式扩增,共扩增出86条DNA带,平均每个引物扩增的DNA带的数目为10.75条,多态性条带数目为53条,占总条带数的61.2%。其中,有5个引物扩增出差异条带和特异条带,并分别能准确地鉴定上述3个树种。应用DPS软件计算供试材料遗传距离界于0.16667～0.80952之间,平均为0.56357。结论]ISSR-PCR方法可以解决红锥、白锥、大叶栎的鉴定问题。
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| 关 键 词： | 壳斗科 红锥 白椎 大叶栎 ISSR 指纹图谱 |
Molecular Identification of Castanopsis hystrix,Castanopsis carlesii and Quercus griffithii Using ISSR-PCR Method |
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| LIU Hai-long,CHEN Xiao-ming,QIN Zi-hai,YANG Kai-tai,LIN Jian-xin,HUANG Jin-shi.Molecular Identification of Castanopsis hystrix,Castanopsis carlesii and Quercus griffithii Using ISSR-PCR Method[J].Agricultural Science ＆ Technology,2011(12):1781-1783. |
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| Authors: | LIU Hai-long CHEN Xiao-ming QIN Zi-hai YANG Kai-tai LIN Jian-xin HUANG Jin-shi |
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| Institution: | 1,2,3 1.Guangxi Zhuang Autonomous Region Forestry Research Institute,Nanning 530002;2.Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China,Nanning 530002;3.Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation,Nanning 530002 |
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| Abstract: | Objective] This research aimed to develop molecular identification method for Castanopsis hystrix,Castanopsis carlesii and Quercus griffithii.Method] DNA fingerprints of C.hystrix,C.carlesii and Q.griffithii were established by using ISSR-PCR method.Cluster Analysis was carried out by using UPGMA method based on Nei’s genetic distances among each individual.Result] Six polymorphic primers were selected from 50 ISSR primers for ISSR-PCR amplification,and totally 86 discernible DNA bands were amplified with 53 polymorphic bands,accounting for 61.2% of the total.The average number of DNA bands amplified by each primer was 10.75.Specifically,totally 5 primers had amplified differential bands and specific bands,which were able to accurately identify C.hystrix,C.carlesii and Q.griffithii.As calculated by DPS v3.01 software,the genetic distances among test materials were ranged from 0.166 67 to 0.809 52,with an average of 0.563 57.Conclusion] ISSR-PCR method can be used to identify C.hystrix,C.carlesii and Q.griffithii effectively. |
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| Keywords: | Fagaceae C hystrix C carlesii Q griffithii ISSR DNA fingerprint |
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