| 冷休克诱导黄河鲇（Silurus asotus）二倍体雌核发育 |
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| 引用本文： | 程果,李学军,乔志刚,石灵,彭新亮.冷休克诱导黄河鲇（Silurus asotus）二倍体雌核发育[J].农业科学与技术,2008,9(2):122-125. |
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| 作者姓名： | 程果 李学军 乔志刚 石灵 彭新亮 |
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| 作者单位： | 河南师范大学生命科学学院,河南新乡453007 |
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| 基金项目： | 河南省科技攻关项目，河南省重点学科建设项目 |
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| 摘 要： | 黄河鲇雌鱼较雄鱼生长快、个体大，养殖全雌黄河鲇是提高经济效益的重要途径。该文首次以黄河鲇为材料，用经过紫外线照射处理后的精子激活成熟的卵子，采用冷休克的疗法人工诱导雌核发育二倍体黄河鲇，
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| 关 键 词： | 冷休克 黄河鲇 二倍体 雌核发育 |
Induction of Gynogenesis of Silurus astus Using Cold Shock |
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| CHENG Guo,LI Xue-jun,QIAO Zhi-gang,SHI Ling,PENG Xin-liang.Induction of Gynogenesis of Silurus astus Using Cold Shock[J].Agricultural Science ＆ Technology,2008,9(2):122-125. |
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| Authors: | CHENG Guo LI Xue-jun QIAO Zhi-gang SHI Ling PENG Xin-liang |
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| Institution: | (College of Life Science, Henan Normal University ,Xinxiang 453007) |
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| Abstract: | Objective] The cold shock method was used to induce diploid gynngenesis of Silurus astus. Mthod] The Silurus astus sperms were irradia-ted by ultraviolet, then conducted for fertilization. The different cold shock starting time and duration were set to observe diploid gynogenesis of Silurus as-tus. Result] Under such inducing condition that ultraviolet irradiated 15 min,cold shock started 5 min after fertilization, and cold shock duartion was 40 min,the survival rate of diploid Silurus astus reached the highest(8.5% ). Conclusion] The experiment laid foundation for culturing new excellent Silu- rus astus variety and accumulated original files for further study of gynogenesis development mechanism. |
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| Keywords: | Silurus asotus Gynogenesis Diploid Cold shock |
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