| 用微卫星标记研究南海四种石斑鱼的遗传变异与种间关系(英文) |
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| 引用本文： | 尹绍武,黄海,骆剑,胡亚丽.用微卫星标记研究南海四种石斑鱼的遗传变异与种间关系(英文)[J].农业科学与技术,2012(2):428-433. |
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| 作者姓名： | 尹绍武 黄海 骆剑 胡亚丽 |
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| 作者单位： | 南京师范大学生命科学学院;海南大学海洋学院 |
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| 基金项目： | Supported by the National High Technology Research and Development Program of China(863Program,No.2007AA091404);the National Key Technology Research and Development Program(2007BAD29B03);A Project Funded by the Academic Program Development of Jiangsu Higher Education Institutions(PAPD)~~ |
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| 摘 要： | 目的]从DNA分子水平揭示我国南海的四种重要经济海水鱼类点带石斑鱼(Epinephelusmalabaricus)、斜带石斑鱼(E.coioides)、鲑点石斑鱼(E.fario)、蜂巢石斑鱼(E.merra)的遗传群体变异水平与种间关系。方法]本研究利用从GenBank中搜索到的21对微卫星引物,对点带石斑鱼、斜带石斑鱼、鲑点石斑鱼及蜂巢石斑鱼的等位基因数A、有效等位基因数Ne、多态信息含量PIC、观察遗传杂合度Ho、期望遗传杂合度He等遗传参数进行检测,并采用UPGMA类聚分析法探讨这四种石斑鱼的种间关系。结果]点带石斑鱼群体的A、Ne、PIC、Ho和He等遗传参数的平均值分别为4.38±1.60、3.69±0.86、0.69±0.08、0.67±0.08、0.72±0.06,斜带石斑鱼群体的为3.88±1.13、3.55±1.04、0.66±0.10、0.68±0.21、0.70±0.08,鲑点石斑鱼群体的为6.00±1.07、4.68±0.65、0.78±0.03、0.73±0.25、0.79±0.03,蜂巢石斑鱼群体的为5.50±1.07、4.58±0.80、0.76±0.05、0.75±0.18、0.78±0.04。结论]综合比较上述数值可知,四个石斑鱼群体的遗传变异程度依次为鲑点石斑鱼群体>蜂巢石斑鱼群体>点带石斑鱼>斜带石斑鱼群体;与其他海水鱼类的遗传变异水平比较,四种石斑鱼的群体遗传变异水平都较高,说明我国这些石斑鱼种类的遗传种质资源尚好。另外,遗传距离与类聚分析表明,点带石斑鱼与斜带石斑鱼遗传距离最近,而斜带石斑鱼与蜂巢石斑鱼的亲缘关系最远。
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| 关 键 词： | 石斑鱼 遗传变异 种间关系 微卫星标记 |
Assessment of Genetic Variability and Inter-specific Relationships among Four Species of Groupers (Genus Epinephelus) from South China Sea Using Microsatellite Markers |
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| Shaowu YIN,Hai HUANG,Jian LUO,Yali HU.Assessment of Genetic Variability and Inter-specific Relationships among Four Species of Groupers (Genus Epinephelus) from South China Sea Using Microsatellite Markers[J].Agricultural Science ＆ Technology,2012(2):428-433. |
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| Authors: | Shaowu YIN Hai HUANG Jian LUO Yali HU |
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| Institution: | 1. College of Life Sciences, Nanjing Normal University, Nanjing 210046, China; 2. College of Ocean, Hainan University, Haikou 570228, China |
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| Abstract: | Objective] The study aimed to evaluate the genetic variability and interspecific relationships among four species of groupers from South China Sea, including E. fario, E. merra, E. malabaricus and E. coioides. Method] Twenty one mircosatellite loci of groupers were selected from GenBank and eight high polymorphic loci were used to further genetic analysis. Result] The mean number of alleles per locus (A), effective number of alleles (Ne), mean polymorphism information content (PIC), observed heterozygosity (Ho) and expected heterozygosity (He) were 4.38±1.60, 3.69±0.86, 0.69±0.08, 0.67±0.08, 0.72±0.06 in E. malabaricus; 3.88±1.13, 3.55±1.04, 0.66±0.10, 0.68±0.21, 0.70±0.08 in E.coioides; 6.00±1.07, 4.68±0.65, 0.78±0.03, 0.73±0.25, 0.79±0.03 in E. fario; 5.50±1.07, 4.58±0.80, 0.76±0.05, 0.75±0.18, 0.78±0.04 in E. merra, respectively. Conclusion] We compared the values above, the order of the genetic variability among these grouper species was E. fario> E. merra > E. malabaricus > E. coioides. We found that the level of genetic variability of these groupers species was relatively higher than that of other marine fish, so their genetic status was good. In addition, the analyisis of genetic relationship showed that E. malabaricus and E. coioides was the closest and it was the farthest between E. merra and E. coioides. |
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| Keywords: | Grouper Epinephelus Genetic variability Species relationships Microsatellite marker |
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