| 津鲢的同工酶分析(英文) |
| |
| 引用本文： | 杨蔷,郝君,鲍迪,梁爱军,金万昆,李崇文,张兴华,董仕.津鲢的同工酶分析(英文)[J].农业科学与技术,2012(7):1571-1574. |
| |
| 作者姓名： | 杨蔷 郝君 鲍迪 梁爱军 金万昆 李崇文 张兴华 董仕 |
| |
| 作者单位： | 天津师范大学生命科学学院,天津市细胞遗传与分子调控重点实验室;国家级天津市换新水产良种场;天津市天祥水产有限责任公司 |
| |
| 基金项目： | 天津市细胞遗传与分子调控重点实验室开放研究基金资助项目;天津市科技支撑计划项目(09zckfnc02100)~~ |
| |
| 摘 要： | 目的]对津鲢进行同工酶分析。方法]使用水平式淀粉凝胶电泳法,对选育品种津鲢进行AAT、EST、α-GPD、GPI、IDH、LDH、MDH、ME、PGM和PROT共10种同工酶及蛋白质的电泳分析,并以购自湖北省荆州市的鲢人工繁殖群体进行比较。结果]以肌肉和肝脏作为检测用组织,共检测出18个基因座位;在津鲢群体中有变异的基因座位为GPI*和PGM*,荆州鲢群体中有变异的基因座位为GPI*;津鲢与荆州鲢的多态基因座位(最高基因频率≤0.99)比例和平均杂合度预期值分别为11.11%、5.56%和0.0150、0.0011,群体间Nei遗传距离为0.00059;2群体GPI*基因座位最高基因频率间的χ2检验结果表明这2个群体间呈极显著差异。结论]为津鲢的大面积推广奠定理论依据。
|
| 关 键 词： | 鲢 同工酶 遗传多样性 |
Isozyme Analysis of Jin Silver Carp (Hypophthalmichthys molitrix Var Jin) |
| |
| Qiang YANG,Jun HAO,Di BAO,Aijun LIANG,Wankun JIN,Chongwen LI,Xinghua ZHANG,Shi DONG.Isozyme Analysis of Jin Silver Carp (Hypophthalmichthys molitrix Var Jin)[J].Agricultural Science ＆ Technology,2012(7):1571-1574. |
| |
| Authors: | Qiang YANG Jun HAO Di BAO Aijun LIANG Wankun JIN Chongwen LI Xinghua ZHANG Shi DONG |
| |
| Institution: | 1. College of Life Science, Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation, Tianjin Normal University, Tianjin 300387, China; 2. National Level Tianjin Huanxin High Quality Fish Farm, Tianjin 301500, China; 3. Tianjin Tianxiang Aquatic Co., Ltd., Tianjin 301500, China |
| |
| Abstract: | Objective] The aim was to carry out isozyme analysis of jin silver carp (Hypophthalmichthys molitrix Var Jing). Method] The isozyme of AAT, EST, α-GPD, GPI, IDH, LDH, MDH, ME, PGM and PROT of muscles and liver in two populations of the silver carp (Hypophthalmichthys molitrix): Jin silver carp (a breed through selective breeding) and artificially propagated population bought from Jingzhou city, Hubei Province were examined by horizontal starch gel electrophoresis. Result] Eighteen loci were observed in two populations. Two loci of GPI* and PGM* in Jing silver carp population and the locus of GPI* in Jingzhou population were polymorphic. The proportions of polymorphic loci (maximum allele frequency≤0.99) of Jing silver carp and Jingzhou populations were 11.11% and 5.56% respectively, expected heterozygosity were 0.015 0 and 0.001 1 respectively. The Nei’s genetic distances were 0.000 59 between two populations. The result of chi-square test of the GPI* gene in two populations showed that their genetic structure has very significant difference. Conclusion] This study provided a theoretical basis for large-scale extension of Jing silver carp. |
| |
| Keywords: | Silver carp (Hypophthalmichthys molitrix) Isozyme Genetic diversity |
| 本文献已被 CNKI 等数据库收录！ |
|
