| 三元杂交猪IL-18全基因的序列测定及分析 |
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| 引用本文： | 曹素芳,李明,王岩,刘长斗,朱赞梅,唐桂芬,肖松云.三元杂交猪IL-18全基因的序列测定及分析[J].农业科学与技术,2009,10(1):89-92. |
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| 作者姓名： | 曹素芳 李明 王岩 刘长斗 朱赞梅 唐桂芬 肖松云 |
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| 作者单位： | 曹素芳,李明,王岩,唐桂芬,肖松云,CAO Su-fang,LI Ming,WANG Yan,TANG Gui-fen,XIAO Song-yun(郑州牧业工程高等专科学校,河南郑州,450011);刘长斗,LIU Chang-dou(郑州先锋动物药业有限公司,河南郑州,450032);朱赞梅,ZHU Zan-mei(河南省大明动物药业有限公司,河南郑州,450027)
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| 基金项目： | 河南省基础与前沿技术研究计划项目，河南省重点科技攻关项目 |
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| 摘 要： | 白细胞介素-18（IL-18）又称γ干扰素诱导因子（IGIF），研究表明IL-18是一种重要的新型免疫佐剂分子。为探讨猪IL-18的免疫佐剂作用，该研究根据已发表的猪IL-18基因序列，设计并合成1对特异性引物，从猪脾脏中直接扩增得到猪IL-18全基因，并进行克隆和序列测定、分析。
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| 关 键 词： | IL-18 三元杂交猪 序列测定 全基因 免疫佐剂 白细胞介素 特异性引物 诱导因子 |
Sequencing and Analysis of Porcine Intrleukin-18 Gene |
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| CAO Su-fang,LI Ming,WANG Yan,LIU Chang-dou,ZHU Zan-mei,TANG Gui-fen,XIAO Song-yun.Sequencing and Analysis of Porcine Intrleukin-18 Gene[J].Agricultural Science ＆ Technology,2009,10(1):89-92. |
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| Authors: | CAO Su-fang LI Ming WANG Yan LIU Chang-dou ZHU Zan-mei TANG Gui-fen XIAO Song-yun |
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| Institution: | CAO Su-fang, LI Ming, WANG Yan, LIU Chang-dou, ZHU Zan-mei, TANG Gui-fen, XIAO Song- yun( 1.Zhengzhou College Zhengzhou 450032; 3 of Animal Husbandry Engineering, Zhengzhou 450011 ; 2. Zhengzhou Xianfeng Animal Pharmaceutical Co., Ltd Henan Darning Animal Pharmaceutical Co., Ltd, Zhengzhou 450027) |
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| Abstract: | Objective] To clone the porcine interleukin-18(IL-18) cDNA and explore the immunological effectiveness of porcine IL-18 as an adjuvant of genetic vaccine. Method] The spleen lymphocytes were isolated from Henan three-way cross-breeding pigs. According to the porcine IL-18 gene in GenBank, a pair of specific primers was designed. The full length cDNA of porcine IL-18 was amplified by RT-PCR. Subsequently, porcine IL-18 cDNA was cloned into pGEM-T vector and sequenced and analyzed. Result] The porcine IL-18 gene demonstrated an open reading frame of 579 bp encoding an inactive precursor protein with 192 amino acids. The precursor protein had no typical hydrophobic signal peptide and cleaved by interleukin-1 beta (IL-1β) converting enzyme (ICE) in caspase-1 splice site; the porcine mature protein had biological activity. After comparing with other porcine IL-18 genes, the nucleotide sequence homology was over 96% and the deduced amino acid homology was more than 98%. Conclusion] A full length procine IL-18 gene was gained. It lays the foundation for porcine IL-18 as an adjuvant of genetic vaccine. |
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| Keywords: | Porcine Interleukin-18 Sequencing Homology |
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