| I群禽腺病毒血清4型广东株的分离鉴定及分子特性 |
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| 引用本文： | 罗洋洋,招丽婵,李群辉,林瑞庆,翁亚彪.I群禽腺病毒血清4型广东株的分离鉴定及分子特性[J].华南农业大学学报,2017,38(6):27-33. |
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| 作者姓名： | 罗洋洋 招丽婵 李群辉 林瑞庆 翁亚彪 |
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| 作者单位： | 1. 华南农业大学 兽医学院,广东 广州,510642;2. 广东温氏食品集团股份有限公司,广东 云浮,527400 |
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| 基金项目： | 国家重点研发计划（2016YFD0501303） |
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| 摘 要： | 目的]对广东地区疑似心包积液–肝炎综合征患病鸡进行腺病毒分离鉴定并研究其分子特征.方法]采集的病料样品,通过鸡胚有限稀释法经SPF鸡胚传代分离纯化病毒,进行致病性试验,并对病毒的hexon、fiber-2、ORF19和ORF29基因进行PCR扩增,分析其分子特征.结果]hexon基因序列分析显示此次分离的毒株为Ⅰ群禽腺病毒血清4型(命名为WM-XC株),SPF鸡接种分离毒株后,致死率达90%,死亡鸡只出现明显的心包积液、肝炎等变化,肝脏病理切片可观察到明显的嗜酸性包涵体,显示分离毒株具有致病性.fiber-2基因序列同经典毒株ON1和KR5相应序列的相似性达96.1%和97.0%,与2015年以来国内分离的毒株序列相似性则达到100%;ORF29序列与2013年国内分离到的毒株JSJ13和JN13的相应序列相比存在33个碱基的缺失;WM-XC分离株与2015年以来国内分离毒株序列相同,但相比经典株ON1和KR-5,缺失ORF19序列.结论]此次从广东地区分离到的血清4型Ⅰ群腺病毒,和近年国内其他地区分离的流行毒株一样,其fiber-2、ORF19和ORF29基因序列与经典毒株差异明显.
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| 关 键 词： | 禽腺病毒 分离鉴定 分子特性 hexon基因 fiber-2基因 致病性 |
| 收稿时间： | 2017/1/21 0:00:00 |
Isolation, identification and molecular characteristics of group I fowl adenovirus serotype 4 from Guangdong, China |
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| LUO Yangyang,ZHAO Lichan,LI Qunhui,LIN Ruiqing and WENG Yabiao.Isolation, identification and molecular characteristics of group I fowl adenovirus serotype 4 from Guangdong, China[J].Journal of South China Agricultural University,2017,38(6):27-33. |
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| Authors: | LUO Yangyang ZHAO Lichan LI Qunhui LIN Ruiqing and WENG Yabiao |
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| Institution: | College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China,Guangdong WENS Foodstuff Group Co., Ltd., Yunfu 527400, China,Guangdong WENS Foodstuff Group Co., Ltd., Yunfu 527400, China,College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China and College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | Objective To isolate and identify the pathogen causing the inclusion body hepatitis (IBH) and hydropericardium syndrome (HPS) in chicken in Guangdong Province, and study its molecular characteristics.Method A virus was isolated from specific pathogen-free(SPF) chicken embryo inoculated with liver suspension from affected chickens. The virus was purified by limiting dilution method and used for pathogenicity test. Hexon, fiber-2, ORF29 and ORF19 genes were amplified by PCR, and analyzed for molecular characteristics.Result Sequence analysis of hexon gene revealed the strain, named as WM-XC, belonged to group I fowl adenovirus serotype 4 (FAdV-4). This isolated strain was pathogenic based on the evidence that the mortality rate of SPF chicken reached 90% after virus inoculation, the hydropericardium-hepatitis syndrome was observed, and acidophilous inclusion body was found in the pathological section of liver. The fiber-2 gene sequence of WM-XC had 96.1% and 97.0% similarities with the corresponding sequences of ON1 and KR-5, and had 100% similarity with fiber-2 genes of fowl adenovirus isolates collected in China since 2015. There was a 33 bp deletion in ORF29 gene of WM-XC compared with JSJ13 and JN13 strains isolated in 2013 in China. Similar to isolates collected in China since 2015, WM-XC lacked ORF19 compared with classical FAdV-4 strain ON1 and KR-5.Conclusion The isolated group I FAdV-4 strain and other isolates collected in China in recent years were distinct from classical FAdV-4 strains in their fiber-2, ORF19 and ORF29 gene sequences. |
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| Keywords: | fowl adenovirus isolation and identification molecular characteristic hexon gene fiber-2 gene pathogenicity |
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