| 家养禽类肠道可培养细菌抗生素抗性的种类、数量和分布 |
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| 引用本文： | 周俊雄,马荣琴,李冬松,田容川,李敏昱,罗艺彬,刘平平,田宝玉.家养禽类肠道可培养细菌抗生素抗性的种类、数量和分布[J].福建农林大学学报(自然科学版),2016(1):56-64. |
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| 作者姓名： | 周俊雄 马荣琴 李冬松 田容川 李敏昱 罗艺彬 刘平平 田宝玉 |
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| 作者单位： | 1. 福建师范大学生命科学学院工业微生物教育部工程研究中心,福建 福州,350108;2. 福州时代中学,福建 福州,350007 |
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| 基金项目： | 国家自然科学基金资助项目(31170108)；福建省杰出青年科学基金项目(2009J06014) |
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| 摘 要： | 采用微生物培养和影印法对家养禽类鸡、鸭和肉鸽肠道可培养细菌抗生素抗性的种类、数量和分布进行了调查.结果表明:在调查的鸡、鸭和肉鸽3种禽类中,肠道可培养细菌抗生素抗性的分布非常普遍,所有测试细菌至少可以抗一种抗生素,抗5种以上抗生素的细菌占测试细菌的比例分别为75.0%、58.9%和97.4%;个别细菌对测试的10种抗生素均具有抗性.在抗生素方面,肠道可培养细菌对萘啶酮酸、盐酸四环素、盐酸克林霉素、磺胺嘧啶和红霉素的抗性分布比较广泛.提取细菌基因组并扩增16S rRNA基因,并对这些细菌进行分子鉴定的结果表明,分离的肠道可培养细菌为埃希氏大肠杆菌.在美国国家生物技术信息中心数据库进一步搜索的结果显示:与肠道可培养细菌同源性大于99%的细菌广泛分布于不同的环境、宿主和病原菌中,表明这些肠道可培养细菌抗生素抗性是一个潜在的环境微生物以及人体病原菌抗生素抗性的传播来源.
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| 关 键 词： | 抗生素 家养禽类 肠道可培养细菌 抗药性 大肠杆菌 |
Diversity and distribution of antibiotic resistance for gut culturable bacteria from domestic poultry |
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| Abstract: | The wide-use, misuse and even abuse of antibiotics in recent years have led to an increase in the resistance of environ-mental and pathogenic microorganisms to antibiotics. In this study, diversity and ecological distribution of antibiotic resistances for culturable intestinal bacteria from domestic poultry, including chicken, duck and pigeon, were investigated using traditional micro-culture and replica plating techniques. Results showed that all the isolated bacteria were resistant to at least 1 of the 10 tested antibi-otics. Proportions of bacteria which were resistant to greater than or equal to 5 antibiotics were 75%, 58.9% and 97.4% for chicken, duck and pigeon, respectively. And 66 out of 192 ( 34%) isolates were resistant to all the antibiotics. Moreover, a variety of bacteri-a, with the highest overall proportion in pigeon and lowest in duck, showed resistance to nalidixic acid, tetracyyline, clindamycin, sulfadiazine and erythromycin. In order to identify bacteria that presentd high and multi-drug antibiotic resistance, 16S rRNA genes of 8 representative strains were amplified and followed by phylogenetic analysis. It turned out that the bacteria were grouped into the branch of Escherichia coli under the family of Enterobacterium. Referring to database from National Center of Biotechnology Informa-tion ( NCBI) , sequences with 99% similarity with the 8 strains were widely distributed in a variety of environment, including soil, animal host and pathogenic bacteria. Results indicated that culturable gut bacteria from domestic poultry was a potential source of an-tibiotic resistance for environmental microbiota and human pathogenic bacteria. |
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| Keywords: | antibiotics domestic poultry culturable intestinal bacteria resistance Escherichia coli |
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