| 仔猪关节炎粪肠球菌生物学特性研究 |
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| 引用本文： | 王亚宾,张祥,胡清林,孟振北,陈丽颖,陈小丽,崔保安.仔猪关节炎粪肠球菌生物学特性研究[J].河南农业大学学报,2011,45(2):183-187. |
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| 作者姓名： | 王亚宾 张祥 胡清林 孟振北 陈丽颖 陈小丽 崔保安 |
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| 作者单位： | 1. 河南农业大学牧医工程学院,河南,郑州,450002
2. 漯河出入境检验检疫局,河南,漯河,450046 |
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| 基金项目： | 河南省重大公益科研攻关项目(81100912300); 漯河市科技计划项目(081203) |
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| 摘 要： | 从病猪肿大的跗关节中分离了1株疑似肠球菌菌株,经过常规方法进行染色特性、培养特性观察以及敏感性和致病试验,利用Vitek-32全自动细菌鉴定系统进行生化特定,并用PCR方法扩增分离株的16S rRNA基因克隆测序,与GenBank上登录的相关菌株及3个粪肠球菌标准菌株进行16S rRNA序列比较、同源性分析并构建系统发...
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| 关 键 词： | 粪肠球菌 仔猪 关节炎 生物学特性 |
Studies on biological characteristics of Enterococcus faecalis caused by piglet's arthritis |
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| WANG Ya-bin,ZHANG Xiang,HU Qing-lin,MENG Zhen-bei,CHEN Li-ying,CHEN Xiao-li,CUI Bao-an.Studies on biological characteristics of Enterococcus faecalis caused by piglet's arthritis[J].Journal of Henan Agricultural University,2011,45(2):183-187. |
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| Authors: | WANG Ya-bin ZHANG Xiang HU Qing-lin MENG Zhen-bei CHEN Li-ying CHEN Xiao-li CUI Bao-an |
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| Institution: | WANG Ya-bin1,ZHANG Xiang1,HU Qing-lin1,MENG Zhen-bei2,CHEN Li-ying1,CHEN Xiao-li1,CUI Bao-an1(1.College of Animal Science and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China,2.Luohe Entry-Exit Inspection and Quarantine Bureau,Luohe 450046,China) |
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| Abstract: | An enterococcus-like isolated was isolated from piglet with lesion tarsal jiont.Morphological and cultural characteristics were observed,and drug sensitivity and piglet challenge tests were performed using the conventional approaches.Biochemical tests for the isolate were carried out by the Vitek-32 automatic microorganism identification system.Moreover,16S rRNA genes of the isolate were amplified by means of polymerase chain reaction(PCR) and the target fragments were cloned and sequenced.A dendrogram was ... |
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| Keywords: | Enterococcus faecalis piglet arthritis biological characteristics |
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