| 小麦纹枯病生防菌株S 024的诱变育种研究 |
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| 引用本文： | 王玲,刘小钟,袁虹霞,邢小萍,李洪连.小麦纹枯病生防菌株S 024的诱变育种研究[J].河南农业大学学报,2006,40(6):622-626. |
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| 作者姓名： | 王玲 刘小钟 袁虹霞 邢小萍 李洪连 |
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| 作者单位： | 河南农业大学植物保护学院,河南,郑州,450002 |
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| 基金项目： | 河南省自然科学基金
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国家粮食丰产科技工程项目 |
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| 摘 要： | 采用5种诱变方法(紫外线、微波、LiCl,LiCl 紫外线、LiCl 紫外线 微波)对出发菌株S 024进行了诱变处理.结果表明,几种诱变方法对S 024的诱变效果差异较大,紫外线(UV)照射和LiCl处理均未能筛选到正突变菌株;微波(MV)处理(50,100 s)可选育出高效菌株,LiCl和UV复合诱变处理的效果最好,共筛选到3个高效菌株(LiU-024-1,LiU-024-4,LiU-024-9),其中LiU-024-4菌株抑菌率比出发菌株S 024提高了34.44%;连续3代转代培养,其产生抗生素的能力基本稳定.用MV对3株高效菌株再进行诱变处理,其抑菌活性基本没有提高.
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| 关 键 词： | 小麦纹枯病 生防菌株S024 诱变育种 |
| 文章编号： | 1000-2340（2006）06-0622-05 |
| 收稿时间： | 2006-05-06 |
| 修稿时间： | 2006年5月6日 |
Mutative Breeding of Strain S 024 for Biocontrol of Wheat Sharp Eye-Spot |
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| WANG Ling,LIU Xiao-zhong,YUAN Hong-xia,XING Xiao-ping,LI Hong-lian.Mutative Breeding of Strain S 024 for Biocontrol of Wheat Sharp Eye-Spot[J].Journal of Henan Agricultural University,2006,40(6):622-626. |
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| Authors: | WANG Ling LIU Xiao-zhong YUAN Hong-xia XING Xiao-ping LI Hong-lian |
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| Institution: | College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China |
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| Abstract: | The biocontrol strain S 024 was induced and mutated by five mutagenic agents,UV-ray,LiCl,microwave,LiCl UV-ray and LiCl UV-ray microwave.The results showed that different mutagenic agents had dissimilar effects on strain S 024 mutation.No positive mutated strains were obtained by UV and LiCl treatments,but microwave treatment had produced effective strain S 024 mutation.However,the effect of multiple mutagenic agents(LiCl UV-ray)was better then single mutagenic agent.Three high-effective strains were obtained.Among them,LiU-024-4 strain had 34.44% enhancement of inhibitory effect on pathogen compared with initial S 024 strain. |
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| Keywords: | Rhizoctonia cerealis biocontrol strain S 024 mutative breeding |
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