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耐冬山茶愈伤组织诱导研究
引用本文:董慧慧,王奎玲,薛秋华,刘庆华.耐冬山茶愈伤组织诱导研究[J].福建林业科技,2007,34(3):135-138,154.
作者姓名:董慧慧  王奎玲  薛秋华  刘庆华
作者单位:1. 莱阳农学院,山东,青岛,266109;福建农林大学,福建,福州,350002
2. 莱阳农学院,山东,青岛,266109
3. 福建农林大学,福建,福州,350002
基金项目:山东省良种工程项目“耐冬山茶种质资源研究”的部分研究内容
摘    要:重点探讨耐冬山茶愈伤组织诱导过程中不同植物生长调节剂、培养基pH值、不同培养基及不同类型外植体对愈伤组织诱导效应的影响。研究结果表明:MS+6-BA 2.0 mg.L-1+2,4-D 0.5 mg.L-1培养基是耐冬山茶愈伤组织诱导的最佳培养基;耐冬山茶愈伤组织诱导培养基pH值以5.5较为合适;耐冬山茶叶柄作为外植体,诱导率和生长状况最佳。

关 键 词:耐冬山茶  组织培养  愈伤组织诱导
文章编号:1002-7351(2007)03-0135-04
修稿时间:2006-11-272007-02-24

The preliminary studies on the callus induction of Camellia japonica "Naidong"
DONG Hui-hui,WANG Kui-ling,XUE Qiu-hua,LIU Qing-hua.The preliminary studies on the callus induction of Camellia japonica "Naidong"[J].Journal of Fujian Forestry Science and Technology,2007,34(3):135-138,154.
Authors:DONG Hui-hui  WANG Kui-ling  XUE Qiu-hua  LIU Qing-hua
Institution:1. Lai yang Agriculture College, Qingdao , Shandong 266109, China; 2. Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
Abstract:The experiment is to study the callus induction effect by using different kinds of hormones,different mediums,pH and different explants,in order to develop the technique systems of tissue culture and rapid propagation for C.japonica "Naidong".The major results as the following: Medium MS+6-BA 2.0 mg·L-1 +2,4-D 0.5 mg·L-1 is optimum for callus induction of C.japonica "Naidong";The pH 5.5 is comparatively suitable for C.japonica "Naidong" callus induction;Using the leafstalk of C.japonica "Naidong" as the explants,the induction rate and the growth is best.
Keywords:Camellia japonica "Naidong"  tissue culture  callus induction
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