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| 油茶SRAP-PCR反应体系的优化 | |
| 引用本文： | 郑婷婷,林 萍,王开良,姚小华,杨水平.油茶SRAP-PCR反应体系的优化[J].林业科学研究,2010,23(2):302-307. |
| 作者姓名： | 郑婷婷 林 萍 王开良 姚小华 杨水平 |
| 作者单位： | 1. 西南大学资源环境学院,重庆,400716;中国林业科学研究院亚热带林业研究所,浙江,富阳,311400 2. 中国林业科学研究院亚热带林业研究所,浙江,富阳,311400 3. 西南大学资源环境学院,重庆,400716 |
| 基金项目： | "十一五"国家科技支撑专题"高产优质油茶香榧新品种选育"(2006BAD01A1706);中国林科院公益性科研基金专项"高油高抗油茶杂交新种质创制"(CAFYBB2008005);中国林科院亚林所公益性科研基金专项"油茶品种分子鉴别系统构建及油茶基因芯片开发"(RISF6804) |
| 摘 要： | Camellia oleifera is one of the important oil tree species in south China, and C. oleifera industry is quickly developed with the support of the national policies in recent years. The disorder of C. oleifera varieties is one of the key issues restricting the development of C. oleifera industry. Because of high polymorphism, good repeatability, less use of DNA and so on, SRAP as a new marker was used in identification of cultivars, analysis of genetic resources and genetic diversity in recent years. In this paper, the orthogonal design was used to optimize SRAP-PCR system for C. oleifera by 5 factors (Mg2+, dNTPs, primer, Taq polymerase, DNA template) and 4 levels, respectively. The data were analyzed by software SPSS V13.0. A suitable SRAP-PCR system (20 μL) was established as: 75 ng DNA template, 1.5 mmol·L-1 Mg2+, 0.15 mmol·L-1 dNTPs, 1U Taq DNA polymerase, 0.4 μmol·L-1 primer, 1×PCR buffer. The result of optimal SRAP-PCR system will provide a foundation for the identification of C. oleifera cultivars. |
| 关 键 词： | 油茶 SRAP 正交设计 体系优化 |
Optimization of SRAP-PCR System for Camellia oleifera |
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| ZHENG Ting-ting,LIN Ping,WANG Kai-liang,YAO Xiao-hua and YANG Shui-ping.Optimization of SRAP-PCR System for Camellia oleifera[J].Forest Research,2010,23(2):302-307. | |
| Authors: | ZHENG Ting-ting LIN Ping WANG Kai-liang YAO Xiao-hua and YANG Shui-ping |
| Institution: | 1.College of Resources and Environment of Southwest China University/a>;Chongqing 400716/a>;China/a>;2.Research Institute of Subtropical Forestry/a>;Chinese Academy of Forestry/a>;Fuyang 311400/a>;Zhejiang/a>;China |
| Abstract: | Camellia oleifera is one of the important oil tree species in south China,and C.oleifera industry is quickly developed with the support of the national policies in recent years.The disorder of C.oleifera varieties is one of the key issues restricting the development of C.oleifera industry.Because of high polymorphism,good repeatability,less use of DNA and so on,SRAP as a new marker was used in identification of cultivars,analysis of genetic resources and genetic diversity in recent years.In this paper,the o... |
| Keywords: | Camellia oleifera SRAP orthogonal design system optimization |
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