| 一种新的白蜡虫寄生蜂体内Wolbachia的wsp基因分子检测及序列分析 |
| |
| 引用本文： | 杨璞,朱家颖,谢正华,李萌,陈晓鸣,陈晓庆.一种新的白蜡虫寄生蜂体内Wolbachia的wsp基因分子检测及序列分析[J].林业科学研究,2011,24(3):340-344. |
| |
| 作者姓名： | 杨璞 朱家颖 谢正华 李萌 陈晓鸣 陈晓庆 |
| |
| 作者单位： | 1. 中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,云南昆明,650224
2. 西南林业大学保护生物学院,云南省森林灾害预警与控制重点实验室,云南昆明650224 |
| |
| 基金项目： | 国家自然基金委青年基金(31000983);云南省应用基础研究基金(2010ZC235)和中国林科院资源昆虫研究所中央级公益性科研院所基本科研业务费专项资金(7-015-3)资助 |
| |
| 摘 要： | 采用Wolbachia的通用引物、A大组和B大组的特异性引物对一种新的白蜡虫寄生蜂--长尾啮小蜂(Aprostocetus sp.)体内Wolbachia的wsp基因进行分子检测,所获得的基因片段分别命名为wApr、wAprB和wAprB,长度分别为620、566和463bp;基因序列分析表明:wApA、wAprB与w...
|
| 关 键 词： | 白蜡虫 长尾啮小蜂 Wolbachia wsp |
| 收稿时间： | 2010/11/25 0:00:00 |
Molecular Detection and Sequence Analysis of wsp Gene from Wolbachia in a New Species of Ericerus pela Parasitic Wasps |
| |
| YANG Pu,ZHU Jia-ying,XIE Zheng-hu,LI Meng,CHEN Xiao-ming and CHEN Xiao-qing.Molecular Detection and Sequence Analysis of wsp Gene from Wolbachia in a New Species of Ericerus pela Parasitic Wasps[J].Forest Research,2011,24(3):340-344. |
| |
| Authors: | YANG Pu ZHU Jia-ying XIE Zheng-hu LI Meng CHEN Xiao-ming and CHEN Xiao-qing |
| |
| Institution: | Research Institute of Resource Insects, Chinese Academy of Forestry; The Key laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming 650224, Yunnan, China;Faculty of Conservation Biology, Southwest Forestry University; Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry; The Key laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry; The Key laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming 650224, Yunnan, China;Research Institute of Resource Insects, Chinese Academy of Forestry; The Key laboratory of Cultivating and Utilization of Resources Insects of State Forestry Administration, Kunming 650224, Yunnan, China;Faculty of Conservation Biology, Southwest Forestry University; Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Kunming 650224, Yunnan, China |
| |
| Abstract: | PCR amplification was employed to detect the infection of Wolbachia on Aprostocetus sp., a new species of parasitic wasps on Ericerus pela, by using the universal primers and specific primers for wsp gene of A and B supergroup. The sequences obtained, were named as wApr, wAprA and wAprB, which sizes were 620, 566 and 463 bp respectively. Sequence analysis found that 4 variable basepairs occurred among the sequences. The phylogenetic analysis of wsp gene showed that Wolbachia in Aprostocetus sp. were classified as Dig group of B-Wolbachia. The sequences of wApr and wAprB were submitted to GenBank and the accession numbers assigned were HQ121415 and HQ121417. |
| |
| Keywords: | Ericerus pela Aprostocetus sp Wolbachia wsp |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《林业科学研究》浏览原始摘要信息 |
| 点击此处可从《林业科学研究》下载免费的PDF全文 |
|
